丁酸钠对乳腺癌细胞MCF-7生长抑制的影响

The inhibitory effect of sodium butyrate on MCF-7 breast cancer cells growth

目的探讨丁酸钠对乳腺癌细胞MCF-7生长的抑制作用。方法将乳腺癌细胞MCF-7常规培养至对数生长期,采用浓度为0、2.5、5.0、10.0mmol/L的丁酸钠处理后,观察对MCF生长的抑制作用。结果浓度为2.5、5.0、10.0mmol/L的丁酸钠处理的MCF-7细胞生长速度明显慢于亲本细胞,随着浓度的增加呈减慢趋势,差异有统计学意义(P<0.05)。浓度为5mmol/L的丁酸钠处理后的MCF-7细胞克隆形成率明显低于MCF亲本细胞,差异有统计学意义(P<0.05)。丁酸钠处理的细胞G1明显高于亲本细胞,S期及G2/M期明显低于亲本细胞,凋亡细胞百分比明显高于亲本细胞,差异有统计学意义(P<0.05)。结论丁酸钠可能是通过诱导MCF-7细胞凋亡而起到逆转其恶性生物学行为的作用。

Objective To investigate the inhibitory effect of sodium butyrate on the growth of breast cancer cells MCF-7. Methods Breast cancer MCF-7 cells were cultured to logarithmic growth phase, they were processed with sodium butyrate, 0, 2.5, 5.0, 10.0mmol/L were the concentration, the growth inhibition of MCF was observed. Results The growth rate of MCF-7 cell processed with the sodium butyrate with the Concentration 2.5, 5.0, 10.0mmol/L was significantly slower than that of the parent cell, the growth rate with increasing concentration was slower, there was the significant difference（P 〈 0.05）. The colony efficiency of MCF-7 cells processed with the sodium butyrate with the concentration 5mmol/L was significantly lower than that of the parental ceils, there was the significant difference （P 〈 0.05）. After processed with sodium butyrate, Cell G1 was significantly higher than the parental cells, S phase and G2 / M phase were significantly lower than the parental cells, the percentage of apoptotic cells was significantly higher than the parental cells, there was the significant difference（P 〈 0.05）. Conclusion Sodium butyrate may be by inducing apoptosis in MCF-7 cells and play a reversal of its role in the malignant behavior.