阿霉素加热化疗对人肝癌细胞HHCC及HepG2的抑制作用研究

Inhibiting effect of doxorubicin heating chemotherapy in proliferation and apoptosis of hepatic carcinoma HHCC and HepG2 cells

目的探讨阿霉素加热化疗对人肝癌细胞HHCC及HepG2的抑制作用。方法选择体外培养对数生长期HHCC及HepG2细胞为研究对象,采用10%胎牛血清培养液配制成细胞密度5×104/mL的单细胞悬液,接种于50mL的培养瓶中,细胞分为3组,分别进行单纯加热、单纯化疗、加热化疗处理;采用MTT法检测细胞增殖抑制率,采用流式细胞仪检测细胞凋亡率。结果 HHCC及HepG2细胞增殖抑制率3组间比较,差异有统计学意义(P<0.05);加热化疗组细胞增殖抑制率明显高于单纯加热组及单纯化疗组(P<0.05);HHCC及HepG2细胞凋亡率三组间差异有统计学意义(P<0.05),加热化疗组细胞凋亡率明显高于单纯加热组及单纯化疗组(P<0.05)。结论加热化疗可明显增强阿霉素对人肝癌细胞HHCC及HepG2的增殖抑制作用,对临床治疗有借鉴意义。

Objective To investigate the inhibiting effect of doxorubicin heating chemotherapy in proliferation of hepatic carcinoma HHCC and HepG2 cells. Methods lnvitro culture HHCC and HepG2 cells in logarithmic growth phase were selected for the study,10% fetal bovine serum medium was used to formulated single cell suspension with cell density of 5×10^4/mL,and then inoculated in the 50mL cultivation bottle. The cells were divided into 3 groups,and given simple heating, simple chemotherapy,heating chemotherapy treatment respectively,cell proliferation was detected by MTT assay,and was apoptosis rate was detected by flow cytometer. Results Proliferation inhibition rate of HHCC and HepG2 cell among the three groups compared,the difference was statistically significant（P 〈 0.05）, inhibition rate of heating chemotherapy group was significantly higher than simple heating group and simple chemotherapy group （P 〈 O.05）;apoptosis rate of HHCC and HepG2 cells of three groups compared,differences was statistically significant （P 〈 0.05）.apoptosis rate of heating chemotherapy group was significantly higher than simple heating group and simple chemotherapy group （P 〈 0.05）. Conclusion Doxorubicin heating chemotherapy can significantly enhance inhibiting effect of doxorubicin on HHCC and HepG2 hepatoma cell, it has reference significance for clinical treatment.