腰痹通胶囊对退变腰椎间盘Ⅱ型胶原含量和TNF-α、IL-1βmRNA表达的影响

Effects of Yaobitong Capsule on the Content of Type II Collagen and Expression of IL-1β and TNF-αmRNA in Rabbit Degenerated Lumbar Intervertebral Disc

目的:观察腰痹通胶囊对兔退变腰椎间盘Ⅱ型胶原含量和TNF-α、IL-1βmRNA表达的影响。方法:采用纤维环针刺法复制兔腰椎间盘退变动物模型。造模成功后,将48只新西兰大白兔随机分为腰痹通、腰痛宁、美洛昔康、模型对照组4组,给药6周,处死后取退变的腰椎间盘,采用免疫组化染色法测定Ⅱ型胶原含量,RT-PCR法检测TNF-α、IL-1βmRNA的表达情况。结果:腰痹通、腰痛宁组Ⅱ型胶原的含量较美洛昔康和模型对照组均有明显提高,相比差异具有统计学意义(F=9.41,P=0.008<0.01)。与模型组比较,腰痹通、腰痛宁、美洛昔康组TNF-α、IL-1βmRNA的表达均下调(F=10.32,P=0.006<0.01);腰痹通、腰痛宁、美洛昔康组TNF-α、IL-1βmRNA的表达比较差异没有统计学意义(F=3.26,P=0.086>0.05)。结论:腰痹通胶囊提高了椎间盘中Ⅱ型胶原的含量,并下调了TNF-α、IL-1βmRNA的表达,减少了炎症介质的合成,这可能是腰痹通胶囊减缓椎间盘退变,治疗椎间盘退行性疾病的机制之一。

Objective：To observe the effects of Yaobitong Capsule on the content of type II collagen and expression of IL-1β and TNF-α mRNA in rabbit degenerated lumbar intervertebral disc. Methods： After successful reproduction of disc degeneration animal model by an annulus needle puncture, 48 New Zealand rabbits were randomly and equally divided into 4 groups, i.e. Yaobitong Capsule group, Yaotongning Capsule group, Meloxicam Capsule group and model control group. Then each group was given the corresponding medicines for 6 weeks. Immuno-histochemical staining method was used to detect the content of type II collagen in the intervertebral disc. The expression of IL-1β and TNF-α mRNA was detected by RT-PCR. Results：Compared with Meloxicam Capsule and model control groups, the content of type II collagen of Yaobitong and Yaotongning Capsule groups were higher （F=9.41, P=0. 008〈0.01）. The expression of IL-1β and TNF-α mRNA of the model control group were higher compared with the other three groups respectively （F= 10.32, P= 0. 006%0. 01） . There was no significant difference in the expression of IL-1β and TNF-α mRNA between the Yaobitong, Yaotongning and Meloxieam groups （F= 3.26, P= 0. 086〉0.05）. Conclusion.. Yaobitong Capsule increases the content of type II collagen of lumbar intervertebral discs, and down-regulates the mRNA expression of IL-1β and TNF-α, thus reduces production of some inflammatory mediators, which might be one of the mechanisms of Yaobitong Capsule treating lumbar disc herniation.