结直肠癌患者外周血Tc17细胞在肿瘤进展中的变化

Changes of circulating Tc17 cells in progression of colorectal cancer

目的:探讨结直肠癌患者外周血Tc17细胞在肿瘤进展中的变化及可能的机制。方法:取54例结直肠癌患者外周血,流式细胞术检测外周血Tc17细胞比例和在不同浓度的IL-1β、IL-6和TGF-β作用下体外培养的PBMCs中Tc17细胞比例;ELISA检测血清中IL-1β、IL-17A、IL-23和IL-6水平。结果:晚期结直肠癌外周血中Tc17细胞比例及IL-1β、IL-17A、IL-23和IL-6水平均高于健康对照(P<0.05)。与早期结直肠癌组相比,晚期结直肠癌组Tc17细胞比例及IL-1β、IL-17A水平明显降低,但IL-6水平明显升高(P<0.05)。早、晚两组IL-23水平差异无统计学意义(P>0.05)。体外试验证实IL-1β或高浓度的IL-6和高浓度的TGF-β能诱导PBMCs中Tc17细胞的分化。结论:IL-1β、IL-23、IL-6和TGF-β可能调控结直肠癌进展中外周血Tc17细胞水平的变化。

Objective：To explore the changes and possible mechanisms of circulating Tc 17 cells in patients with colorectal cancer （ CRC） following disease progression .Methods：The peripheral blood were collected from 54 patients with colorectal cancer . the proportions of Tc17 cells in peripheral blood mononuclear cells （PBMCs） and cultured PBMCs treated by IL-1β, IL-6 and TGF-βin different concentrations were determined by flow cytometry;the levels of IL-1β, IL-17A, IL-23 and IL-6 in sera were measured by ELISA.Results：The proportions of Tc17 cells and the levels of IL-1β, IL-17A, IL-23 and IL-6 were significantly higher than those in healthy controls （P〈0.05）.Compared with early CRC, the proportions of Tc17 cells and the levels of IL-1β, IL-17A in advanced CRC were significantly decreased, but the level of IL-6 was significantly increased （P〈0.05）.The difference of IL-23 level between early and late groups was not significant （P〉0.05）.In vitro experiments confirmed that IL-1βor high concentrations of IL-6 and TGF-βcould significantly increase the number of Tc 17 cells in PBMCs.Conclusion： The changes of circulating Tc 17 cells in the progression of colorectal cancer are possibly modulated by IL-1β, IL-23, IL-6 and TGF-β.