氮代谢调控相关蛋白质泛素化修饰筛选

Ubiquitination Detection of NCR Related Permeases and Regulators

通过对氮代谢相关透性酶及调控因子泛素化修饰的筛选,为通过改造蛋白质泛素化修饰来解除氮代谢阻遏效应提供参考。对此前建立的一种蛋白质泛素化快速检测方法进行了改造和发展,进一步增强了阳性子的荧光信号,并使得该方法可以用于定量分析,从而使泛素化检测的结果更为可靠。对氮代谢物阻遏效应密切相关的10个蛋白质的检测结果表明,其中5个受到泛素化修饰,4个不能受到泛素化修饰,1个不能确定是否受到泛素化修饰。除建立了一种可靠的蛋白质泛素化检测方法,也为通过蛋白质泛素化调控途径解除氮代谢物阻遏效应提供了思路。

To give support to release the nitrogen catabolite repression （NCR）,by rebuiling the ubiquitination system,the ubiquitination of NCR related permeases and regulators was detected.A fast ubiquitination detection procedure established before was modified and developed,which enhanced the fluorescence of the positive control and could quantify the fluorescence signal.The fluorescence quantification made this procedure more reliable.It was demonstrated that 5 of the NCR related proteins were ubiquitinated,4 were not ubiquitinated and 1 was not determined.We not only established a reliable ubiquitination detection procedure,but also gave a idea to release NCR through the ubiquitination modification procedure.