鹿血中甾体化合物的分离与结构鉴定

Extraction and Structural Identification of Steroidal Compounds from Deer Blood

利用乙醇对鹿血的有效成分进行提取,经MCI柱层析粗分,再经ODS反相色谱柱反复分离,得到3个化合物Ⅰ、Ⅱ、Ⅲ,通过1HNMR、13CNMR等方法鉴定出Ⅰ为△4,14-雄烯-2醇-28,30环戊二烯庚酸酯-3酮;Ⅱ为△4,14-雄烯-2,19醇-28,30环戊二烯庚酸酯-3酮;Ⅲ为△4,14-雄烯-2醇-27,29环戊二烯己醚-3酮。结果显示,3个化合物均为雄性激素衍生物,这3个雄性激素衍生物在鹿血中属首次发现。

Effective components were extracted from deer blood by ethanol in this study,firstly separated through the MCI column,and then purified by ODS reversed phase chromatographic column,repeated separation.Finally,three kinds of compounds were extracted.,their structures were identified by 1HNMR,13CNMR et al：the compound Ⅰ was △4,14-androstene-2 alkanol-28,30 Cyclopentadiene heptanoic acid ester-3 ketone;the compound Ⅱ was △4,14-androstene-2,19 alkanol-28,30 Cyclopentadiene heptanoic acid ester-3 ketone;the compound Ⅲ was /4,14-androstene-2 alkanol-27,29 Cyclopentadiene hexyl ether-3 ketone.The results shown that three compounds are all male hormone derivatives,they are all firstly reported in deer blood.