SOX1对鼻咽癌细胞化疗敏感性的影响

Effect of SOX1 gene overexpression on chemotherapeutic sensitivity of nasopharyngeal carcinoma cells

目的 观察SOXl基因过表达对鼻咽癌细胞化疗敏感性的影响.方法 采用脂质体Lipofectamin2000介导的方法,将SOXl基因转入鼻咽癌细胞CNE2和HONE1中,RT-PCR和Western blot法检测转染空载体对照组及转染SOX1过表达组鼻咽癌细胞中SOX1基因的表达,并用MTT法检测两组鼻咽癌细胞对化疗药物的敏感性.结果 RT-PCR和Western blot法表明,转染过表达组鼻咽癌细胞的SOX1基因的mRNA及蛋白表达水平均较对照组明显增强,MTT法结果表明过表达SOX1的鼻咽癌细胞对化疗药物顺铂的敏感性显著提高（P＜0.05）.结论 转染外源性SOX1并使之在鼻咽癌细胞中过表达,能提高CNE2和HONE1细胞对化疗药物的敏感性.

Objective To explore the effect of overexpressed SOX1 gene upon chemotherapeutic sensitivity of nasopharyngeal carcinoma cells.Methods Via Lipofectamin2000,SOX1 gene was transfected into NPC cell lines CNE2 and HONE 1.Cellular SOX1 expression was detected by reverse transcriptionpolymerase chain reaction （RT-PCR） and Western blot analysis.MTT was employed to investigate the sensitivity of DDP treatment between NPC cells with or without SOX1 overexpression.Results RT-PCR and Western blot results demonstrated that SOX1 mRNA and protein level of CNE2-SOX1 and HONE1-SOX1 cells were significantly higher than the two NPC cell lines in control group.MTT result showed that NPC cell lines which overexpressed SOX1 were more sensitive to DDP treatment than that in control group （P 〈 0.05）.Conclusion Transfection of extrinsic SOX1 gene contributes to its overexpression in CNE2 and HONE1,which can increase the chemotherapeutic sensitivity of the two NPC cell lines.