白百利烟草愈伤组织细胞细胞分裂素结合蛋白的研究

STUDY ON CYTOKININ BINDING PROTEIN FROM CELL OF BAIBAILI TOBACCO CALLUS

利用BA-Sepharose 4B亲和层析技术丛白百利烟草(Nicotiana tabacum Baibaili)愈伤组织细胞分离提纯了分子量为4400±100道尔顿的细胞分裂素结合蛋白(CB-蛋白)。在细胞表面、核糖体、线粒体、叶绿体和细胞核上以及在细胞液中都有CB-蛋白存在,而核糖体上的CB-蛋白含量量高。探讨了CB-蛋白的功能。

Cytokinin binding proteins (CB-protein) which moleculer weight arc about 4400±100 dalton from cell of Baibaili tobacco callus was purified by affinity chromatography on benzylaminopurin-Knked sephrosc 4B column. There is CB-protein on cell surface, ribosome, mitochondria, chloroplast and nucleus as well as in cell sap; amount of CB-protein on ribosome is the highest among organellcs. We probed into the function of CB-protein.