HPLC测定紫丁香叶中羟基酪醇含量

Determination of Hydroxytyrosol in Syringa oblata Lindl. leaf by HPLC

目的：建立一种测定紫丁香叶中羟基酪醇含量的方法。方法：采用HPLC，色谱柱为DikmaTech-nologies C18柱（250mm ＆#215;4．6mm，5μm），流动相为甲醇-水溶液（70∶30），检测波长为221nm。结果：羟基酪醇在3．03～48．40μg/mL内呈良好的线性关系，平均加样回收率为98．3％（ RSD＝1．6％）。结论：本方法简便易行、准确可靠，可为紫丁香叶药材及制剂的质量评价提供依据。

Objective：To establish a method for the determination of Hydroxytyrosol in Syringa oblata Lindl.leaf.Meth-ods：The determination was developed on C 18 column.Methanol -water was used as mobile phase and the detective wavelength was set at 221nm, respectively.Results：The linear ranges of Hydroxytyrosol were 3.03~48.40μg/mL（r=0 .999 8 ） , respectively .The average recovery was 98 .3%（ RSD=1 .6%） , respectively .Conclusion：The method is simple and accurate , which could be used for the quality control of Syringa oblata Lindl.leaf.