摘要
建立了液相色谱-四级杆串联质谱法测定氟尼辛葡甲胺含量的方法,对其检验条件进行了优化。采用Agilent eclipse plus C18色谱柱分离,用体积浓度0.1%甲酸溶液、0.1%甲酸乙腈溶液作为流动相梯度洗脱,电喷雾正离子(ESI+)模式电离,多反应监测(MRM)模式检测,外标法定量。该方法操作灵敏度高,重复性好。
The determination method of Flunixin meglumine by LC-MS/MS was established and the determination con-dition was optimized. Flunixin meglumine was separated with C18 （2.1 mm ＆#215;100 mm, 3.5 μm） column using 0.1%formic acid aqueous solution 、 0.1% formic acid acetonitrile solution as a gradient elution mobile phase. It was ionized under positive ion pattern by Electrospray Ionization Source （ESI＋）, detected by Multiple Reaction Monitoring （MRM） and quantified by external standard method.
出处
《食品工程》
2014年第2期55-57,64,共4页
Food Engineering
基金
国家质检总局科技计划项目"食品中氟尼辛葡甲胺的串联液质色谱方法的研究"(2010QK263)
关键词
液相色谱
-
四级杆串联质谱法
电喷雾
氟尼辛葡甲胺
测定
liquid chromatographic-tandem mass spectrometric method （LC-MS/MS）
electrospray
flunixinmeglumine
determination
