miR-130a-3p下调CYLD表达对乳腺癌MCF-7细胞功能的影响

The effect of miR-130a-3p on function of human breast cancer cell line MCF-7 by down-regulating the expression of CYLD

目的:探讨miR-130a-3p对人乳腺癌MCF-7细胞功能的影响及其可能的靶向基因。方法:应用脂质体介导方法将miR-130a-3p模拟物(实验组)或对照模拟物(对照组)转染MCF-7细胞,分别采用MTT方法和细胞划痕实验检测细胞增殖和迁移能力变化,印迹法检测细胞中miR-130a-3p靶基因圆柱瘤基因(CYLD)的表达。结果:与对照组比较,实验组MCF-7细胞的增殖和迁移能力增强(P<0.05),细胞中CYLD蛋白表达水平明显下调(P<0.05)。结论:miR-130a-3p可能通过靶向调节CYLD蛋白表达增强人乳腺癌MCF-7细胞的增殖和迁移。

Objective：To investigate the effect of miR-130a-3p on the function of human breast cancer cell line MCF-7, and to explore its possible target gene. Methods： miR-130a-3p mimics or its negative control （miR-130a-3p mimics-NC） was transfected into MCF-7 cells by lipofectamine. The capacities of proliferation and migration of MCF-7 cells were evaluated by MTT and Scratch assay, respectively. The expression of CYLD which was predicted as a target gene in MCF-7 cells was determined by Western blotting. Results：The capacities of proliferation and migration in MCF-7 cells transfected with miR-130a-3p mimics were signiifcantly promoted and the expression level of CYLD was down-regulated when compared with those in MCF-7 cells transfected with miR-130a-3p mimics-NC（P〈0.05）. Conclusion：The miR-130a-3p expression can promote the proliferation and migration of human breast cancer line MCF-7 by down-regulating the expression of CYLD.