乌司他丁对脑缺血再灌注大鼠脑组织IL-17的影响

Effect of Ulinastatin on IL-1 7 in Brain Tissues of Rats with Cerebral Ischemia-reperfusion Inj u-ry

【目的】研究乌司他丁（UTI）对脑缺血再灌注损伤的保护作用及机制。【方法】雄性 SD大鼠72只，体质量为200～250 g，10～12周龄，随机分三组：假手术组，模型组和 UTI治疗组。其中模型组和 UTI治疗组均按大脑中动脉线栓法建立缺血再灌注模型，缺血时间为60 min。假手术组只行颈部手术，不行血管栓塞。UTI治疗组术后每日腹腔注射 UTI（10000 U/kg），对照组和假手术组每日注射等量生理盐水，应用Petullo评分在缺血再灌注之后 d3、d7及 d14来评价3组大鼠神经功能，应用免疫组织化学染色及免疫印迹western blot法在缺血再灌注之后 d3、d7及 d14检测3组大鼠白细胞介素-17（IL-17）的表达情况，观察 UTI对脑缺血再灌注大鼠的神经功能及脑组织 IL-17的影响。【结果】与模型组相比，UTI治疗组 petullo评分在 d3（2．87±0．25）、d7（2．20±1．52）与 d14（2．00±0．50）时与模型组（d3：5．00±0．91、d7：4．87±0．62、d14：4．50±0．81）相比差异均有显著性（P＜0．05）；UTI治疗组与模型组相比，IL-17表达明显降低，其中 d3、d7和 d14的差异均有统计学意义（P＜0．05）。【结论】UTI可能通过降低 IL-17表达减轻大鼠缺血再灌注后神经功能缺损程度。

[Objective]To explore the protective effect and mechanism of ulinastatin（UTI）against cerebral ischemia-reperfusion injury.[Methods]A total of 72 male SD rats weighed 200~250g and aged 10~12 weeks were randomly divided into 3 groups including sham operation group,model group and UTI-treated group. The model of cerebral ischemia-reperfusion inj ury in model group and UTI-treated group were made by occlu-ding middle cerebral artery with thread,and ischemic time was 60min.Sham operation group only underwent neck surgery without occluding the vessel. UTI-treated group received intraperitoneal inj ection of UTI （10000U/kg）daily.The model group and sham group received the injection of isovolume normal saline daily. Petullo score was used to evaluate nervous function of 3 groups at 3d,7d and 14d after ischemia-reperfusion. Immunohistochemistry and Western blot were used to detect the expression of interleukin-17（IL-17）in rats of 3 groups at 3d,7d and 14d after ischemia-reperfusion.The effect of UTI on nervous function and IL-17 in brain tissues of rats with cerebral ischemia-reperfusion was observed.[Results]There were significant differ-ences in Petullo scores at d3 ,d7 and d14 between UTI-treated group and model group[（2.87±0.25）vs.（5.00 ±0.91）,（2.20±1.52）vs.（4.87±0.62）,（2.00±0.50）vs.（4.50±0.81）]（P〈0.05）.Compared with the model group,the expression of IL-1 7 in UTI-treated group was obviously decreased,and there were signif-icant differences in the expression of IL-17 at d3 ,d7 and d 14 between two groups（P〈0.05）.[Conclusion]UTI may alleviate neurologic impairment after cerebral ischemia-reperfusion by decreasing the expression of IL-17.