摘要
NAC转录因子是植物特有的具有多种生物功能的一类重要转录因子,广泛参与植物生长发育以及生物与非生物逆境应答等。本文利用生物信息学手段,结合大豆基因组数据库及NCBI数据库,识别、筛选并获得了大豆NAC家族基因的蛋白序列,并对其进化关系、在基因组上的定位分布以及理化特性进行了预测和分析。结果显示具有NAC结构域的152条大豆氨基酸序列认为是假定NAC蛋白质,共分为10个亚家族,连锁群定位结果发现大豆的20条染色体上均有NAC转录因子的分布,其中第12号染色体上分布最多。不同亚族间的大豆假定NAC蛋白质理化特性存在一定的差异,无规则卷曲和α-螺旋是蛋白质二级结构最大量的结构元件,并散布于整个蛋白。本研究结果将为大豆NAC基因家族的进一步功能分析奠定重要的研究基础。
Genes containing the NAC domain( NAC family genes)are plant-specific transcriptional regulators and expressed in various developmental stages and tissues. We performed a bioinformatics analysis of NAC family genes in soybean. Based on bioinformatics methods, taking advantage of soybean database combined with public database (NCBI), we identified 152 NAC proteins from soybean genome. NAC domains from both predicted and known NAC family proteins were classified into ten subgroups by sequence similarity. Further genetic mapping of NAC genome localization found that they distributed on 20 chromosomes which the twelfth chromosome exist the most. We also predicted and analyzed their amino acid composition, physical and chemical characteristics, as well as secondary structures. The research found that the number of amino acid and hydrophobic of amino acid sequences in different subfamilies presented some differences. Meanwhile, the results of secondary structure predicted that the main composition of 152 predicted NAC proteins among them were random coil and alpha helix. The results of this investigation could definitely provide a significant foundation for further research on the function analysis of soybean NAC gene family.
出处
《大豆科学》
CAS
CSCD
北大核心
2014年第3期325-333,共9页
Soybean Science
基金
教育部科学技术研究重点项目(212049)
教育部高等学校博士学科点专项科研基金(20102325120002)
转基因生物新品种培育重大专项(2013ZX08004-003)