转AtCBF4基因大豆株系的抗旱性评价

Drought Resistance Evaluation of AtCBF4 Transgenic Soybean

干旱胁迫条件下考察转AtCBF4基因大豆株系的光合特性(净光合速率、气孔导度、胞间CO2浓度、蒸腾速率、瞬时水分利用率)、生理特性(脯氨酸、丙二醛、可溶性糖)以及产量性状(株高、节数、单株荚数、单株粒数、百粒重)的表现,综合评价7个株系之间的抗旱性差别。结果表明:转AtCBF4基因大豆叶肉细胞中脯氨酸的含量和可溶性糖含量与受体相比较高,丙二醛含量增幅较小,光合生产能力和产量性状表现优于受体。综合结荚期和鼓粒期表现,所有转基因株系耐旱性均强于非转基因受体,其中HTCB59-5、HTCB59-2和HTCB59-4具有较强的耐旱能力。

In this paper,we explored the performance of transgenic soybeans in photosynthetic characteristics of soybean （net photosynthetic rate, stomatal conductance, CO2 concentration intercellular, transpiration rate, instantaneous water use efficiency）, physical characteristics （ Pro, MDA, soluble sugar） and yield traits （ plant height, number of sections, the number of pods per plant, seed number per plant, seed weight）under drought stress. Comprehensive evaluate the difference in drought-tolerance among 7 soybean lines. Compared of transgenic lines and normal lines, the research showed that the contents of soluble sugar （WSS） and free proline （Pro）from transgenic lines were higher than normal ones,while less increment of Malondialdehyde （MDA） appeared. The transgenic lines had little change in photosynthetic characteristics and yield traits from before to under drought resistance. All above showed that compared with the normal, all transgenic soybeans have more adjustment for water deficit in pod stage and pod-filling stage. HTCB59-5, HTCB59-2 and HTCB59-4 have more drought tolerance than others.