顶空固相微萃取-气相色谱-质谱法分析玉兰花的挥发性成分

Analysis of Volatile Components in Magnoliaby HS-SPME-GC/MS

采用顶空固相微萃取(HS-SPME)和气相色谱-质谱联用(GC-MS)技术,对白玉兰花和黄玉兰花的挥发性成分进行分析。两种玉兰花共鉴定出91种挥发性成分,其中共有成分检出31种。白玉兰花的主要挥发性成分为2-甲基-丁酸甲酯(42.04%)、丙酸甲酯(13.89%)、苯乙醇(4.86%)、3,7-二甲基-1,6-辛二烯-3-醇(4.61%)和石竹烯(3.80%);黄玉兰花的主要挥发性成分为3,7-二甲基-1,6-辛二烯-3-醇(41.34%)、安息香酸甲酯(9.68%)、4-(2,6,6-三甲基-2-环己烯-1-基)-2-丁酮(7.93%)、苯乙醛(6.70%)、(E)-4-(2,6,6-三甲基-1-环己烯-1-基)-3-丁烯-2-酮(4.29%)、β-月桂烯(4.06%)和D-苧烯(3.55%)。对于萘(樟脑)类化合物,白玉兰花测得的相对百分比为5.20%,黄玉兰花未检出;对于甲酯类化合物,白玉兰花测得的相对百分比为62.10%,黄玉兰花测得的相对百分比为11.60%;对于醇类化合物,白玉兰花测得的相对百分比为9.47%,黄玉兰花测得的相对百分比为42.70%;对于烯烃类(含萜烯)化合物,白玉兰花和黄玉兰花测得的相对百分比分别为17.80%和10.30%。研究结果显示,两种玉兰花的挥发性成分及其相对百分比存在很大差异。

Using headspace solid-phase mieroextraction （HS-SPME） and gas chromatography-mass spectrometry （GC-MS）, the volatile components in Magnolia denudate （M. denudate） and Magnolia champaca （M. champaca） were analyzed. Ninety one volatile components were identified, among which 31 were common in both flowers. The major volatile components of M. denudate were methyl 2-methyl-butanoate （42. 04 %）, methyl propionate （13. 89 % ）, phenyl ethyl alcohol （4.86%）, 3,7-dimethyl-1,6-octadiene-3-ol （4.61%） and earyophyllene （3.80%） ）. The major volatile components of M. champaca were 3,7-dim- ethyl-1,6-octadiene-3-ol （41.34%）, methyl benzoate （9. 68%）, 4-（2,6,6-trimethyl-2-cyclohexerr-l-yl）-2-butanone （7.93%）, benzene.acetaldehyde （6. 70 % ）, （E） -4- （2,6,6-trimethyl-l-eyclohexen- l-yl） -3-buten-2-one （4. 29 % ）, β-myrcene （ 4, 06 % ） and D-limonene （3. 55 %）, The relative content of naphthalene （camphor） compounds was 5.20% in M. denudate ~ but these compounds were not detected in M. champaca. The relative content of methyl ester compounds was 62.10% in M. denudate and 11.60% in M. charnpaca; the relative content of alcohol compounds were 9.47% in M. denudate and 42.70% in M. champaca. The relative content of olefin compounds, including terpene, were 17.80% in M. denudate and 10.30% in M. champaca. This study indicated that the volatile components and their relative contents differed significantly in two Magnolia flowers.