抗多种致病菌的特异性卵黄免疫球蛋白的制备研究

The Manufacture of Composite Egg Yolk Immunogfobulin IgY against Multiple Pathogenic Bacteria

目的:探讨一种新的获得IgY多克隆抗体的方法和效果。方法:用三种常见的细菌病原体金黄色葡萄球菌(SA)、大肠埃希氏菌(EC)、铜绿假单胞菌(PA)制备复合抗原,并经过灭活后免疫来亨蛋鸡产生IgY多克隆抗体。从免疫后的蛋中提取出IgY多克隆抗体,ELISA法测IgY抗体特异性及浓度滴度。SA、EC、PA三种标准菌株体外抑制实验检测IgY抗体抑菌效果。结果:用SA、EC、PA复合抗原成功地免疫来亨蛋鸡产生IgY多克隆抗体,ELISA法检测抗体浓度滴度为100,000;IgY抗体明显抑制SA、EC、PA三种标准菌株的生长。结论:SA、EC、PA三种细菌复合抗原可以刺激来亨蛋鸡产生高浓度滴度抗体IgY,该抗体能明显抑制SA、EC、PA三种标准菌株的生长。

Objective： To explore a novel method of manufacturing polyclonal IgY antibody. Methods： Utilizing three types of common pathogenic bacterial, including Staphylococcus aureus （SA）, Escherichia coli （EC）, Pseudomonas aeruginosa （PA）to immunize laying hens to produce antibody.Using the ELISA to determine the specific and the antibody titer of the polyclonal IgY.Bacteriostatic experiment was used to observe the antibacterial activity of polyclonal IgY in vivo. Result： The complex antigen of the SA, EC, PA was successfully made. The polyclonal IgY can binds specifically to the three antigens and multiple antigen, the titer of the polyclonal antibody was 100,000. Antibacterial activity experiments showed that polyclonal IgY from chickens could significantly inhibit the three types of standard strains. Conclusions： Using the three bacteria multiple antigens to immunize the hens can induce high titer of polyclonal IgY. This polyclonal IgY could significantly restrain the three types of bacteria growth in vitro.