人疱疹病毒6型感染与神经胶质瘤关系的初步研究

A preliminary study on the relationship of human herpes virus 6 infection and glioma

目的探讨人疱疹病毒6型（human herpesvirus-6，HHV-6）感染与神经胶质瘤的关系。方法将辽宁省肿瘤医院神经外科2011年6月-2013年5月收治的神经胶质瘤患者45例纳入病例组，将同期该医院收治且已排除神经胶质瘤的脑外伤患者45例作为对照组。分别采用巢式聚合酶链式反应（nested polymerase chain reaction，Nested—PCR）法检测两组研究对象人病变脑组织样本中HHV-6序列；采用免疫组化染色法检测两组研究对象人病变脑组织样本中HHV-6抗原的表达。结果病例组HHV石DNA阳性率为31．11％，对照组HHV-6DNA阳性率为6．67％（χ2=8．755，P=0．003）。病例组HHV-6早期抗原041表达阳性率为22．22％，对照组HHV-6早期抗原p41表达阳性率为0．00％（χ2=11．250，P=0．001）。病例组HHV-6DNA阳性率、HHV-6抗原阳性率均高于对照组，组间差异有统计学意义（P〈0．05）。结论神经胶质瘤患者和非神经胶质瘤脑外伤患者病变脑组织中HHV石感染率有差异，据此推断HHV-6感染在神经胶质瘤的发生和发展过程中起到一定的作用。

Objective To study the relationship of human herpes virus-6 （HHV-6） infection and glioma. Methods A total of 45 neurosurgery glioma patients who admitted to Shenyang tumour hospital from June 2011 to May 2013 were included in the case group, while another 45 cases with traumatic brain injury glioma were enrolled in the control group. Nested-PCR was used to detect HHV-6 DNA, immunohistochem- istry method was used to detect the expression of HHV-6 in two groups. Results The HHV-6 DNA positive rate was 31.11% （14/45） and 6. 67% （3/45） in case group and control group, respectively. There was statistical difference between them（χ2= = 8. 755, P = 0. 003 ）. The positive rate of p41was 22. 22% （10/45） in case group, while no p41 antigen was checkout from control group, there was statistical difference between them （χ2= = 11. 250, P =0. 001 ）. Conclusions HHV-6 infection play a part in the occurrence and devel- opment of glioma.