甘草饮片、药对以及四君子汤中药效组分的测定

Determination of Active Components Alignments from Licorice Single Herb,Pair Herb and Sijunzi Decoction

目的:以内蒙古野生炙甘草单味药、药对、复方为研究载体,测定甘草中4种药效组分含量,探索药效组分变化规律。方法:采用单味药、药对配伍、复方配伍组分-液相色谱综合分析法。结果:药效组分甘草苷-甘草素-甘草酸-异甘草苷在单味药(内蒙古野生炙甘草)中含量为(12.14±0.31),(3.25±0.19),(38.75±1.77),(1.4±0.25);在药对(炙甘草-人参)中含量为(2.87±0.04),(0.74±0.02),(6.42±0.18),(0.37±0.03);在药对(炙甘草-白术)中含量为(5.99±0.01),(2.16±0.09),(22.4±1.8),(0.77±0.02):在复方(四君子汤)中含量为(2.99±0.03),(0.81±0.04),(10.05±0.31),(0.32±0.03)。结论:甘草中4中药效组分在单味药、药对、复方中含量存在一定差异,为进一步完善甘草药材质量的制定提供新思路和科学依据。

Objective：To explore regularity of the active components alignments （ACA）through determination of the ACA in inner Mon-golia wild licorice single herb,herb-herb and prescription.Methods：The ACA were determined by reversed phase high-performance liq-uid chromatography （RP-HPLC）in single herb,herb-herb and prescription.Results：The contents of liquiritin-liquiritigenin-glycyrrhizin acid-Isoliquiritin in single liquorice herb were（12.14 ±0.31）,（3.25 ±0.19）,（38.75 ±1.77）,（l1.4 ±0.25;in herb-herb （honey-fried licorice-ginseng）were（2.87 ±0.04）,（0.74 ±0.02）,（6.42 ±0.18）,（0.37 ±0.03）;in herb-herb（honey-fried licorice - big-head atractylodes rhizome ）were （5.99 ±0.01）,（2.16 ±0.09）,（22.4 ±1.8）,（0.77 ±0.02）;in prescription were（2.99 ±0.03）, （0.81 ±0.04）,（10.05 ±0.31）,（0.32 ±0.03）.Conclusion：There are some differences in the content of liquiritin- liquiritigenin-glycyrrhizic acid-Isoliquiritinin in single herb,herb-herb and prescription,which provides scientific basis and a new way for the establish-ment of improving the quality of licorice.