高效液相色谱法测定脂降宁片中葛根的含量

High Performance Liquid Chromatography Method for Determining the Content of Fat Drop nNing in the Puerarin

目的建立脂降宁片中葛根的含量测定方法。方法采用HPLC法,以乙腈-水(11:89)为流动相,迪马-platisil ODS C18(5μm,250×4.6mm)色谱柱,流速1.0ml/min,柱温30℃,进样量10μl,检测波长250nm。结果葛根素在0.02582μg^0.7746μg的范围内线性关系良好,r=0.9998,加样回收率为99.7%,RSD=1.1%(n=6),阴性样品无干扰。3批样品含量分别为1.93mg/g、0.584mg/g、0.411mg/g。结论此方法专属性强,准确,简便,可作为脂降宁片中葛根含量的测定方法。

Objective To establish fat drop ning in the puerarin content determination method.Methods HPLC method was used to acetonitrile-water（9） as mobile phase,diplatisil ODS C18（250×4.6mm,5microns） chromatographic column,flow rate of 1.0ml/min,the column temperature 30℃,sample quantity 10μl,detection wavelength of 250nm.Results Ppuerarin in 0.02582～0.02582μg inside the range of sex good,r=0.9998,the recovery was 99.7%,RSD=1.1%（n=6）,the negative samples without interference.3 batches of samples content were 1.93mg,0.584mg/g and 0.411mg/g.Conclusion This method is specific,accurate,simple,can be used as a fat drop ning in the method for determination of the content of puerarin.