利用POX同工酶测定谷子(粟)品种对黑穗病的抗性

Study on Using Peroxidase Isoenzymes to Determine Resistance to Kernel Sumt of Foxtail Millet

采用箱栽方法种植 12个抗病性不同的谷子品种 ,分析了芽苗过氧化物酶 (POX)活性及同工酶的变化。结果证明 ,对黑穗病免疫的品种接菌后POX活性平均提高 2 2 8%。越是易感病的品种接菌后POX活性提高得越多。感病品种平均提高 71 6 % ,高感品种平均提高 95 0 %。同工酶酶谱 ,免疫品种接菌后不变 ,越是易感病的品种接菌后酶谱变化越大。二维排序 ,免疫品种接菌与不接菌的两个点落在同一点上或附近 ,d值平均为 3 1。易感病品种的两点相去甚远 ,其中 ,感病品种平均d值 12 2 ;高感品种平均d值 13 2。采用芽苗POX同工酶测定谷子品种抗黑穗病性 。

Peroxidase(POX) activity and isoenzyme electrophoregrams of shoots on 12 millet varieties with different resistance cultivated in plastic boxes were analysed.The results showed that after disease germ inoculation,POX activity of the immune varieties had a mean increase of 22 8%,and for sensitive varieties,the higher the sensitiveness,the higher the POX activities they got.The disease-sensitive varieties increased an average of 71 6%,and high disease-sensitive varieties increased a mean of 95 0%.For the isoenzyme zymogram of the immune ones no changes observated,while for those disease-sensitive ones,the higher the sensitiveness,the more zymogram changed.In comparation of 2-dimension ordination,two electrophoregram points of immune varieties,one of disease germ inoculated and one of the control,were at the same position,or close to each other,and average d value was 3 1 While those for the disease-sensitive ones were far apart.The mean d value of sensitive ones were 12 2 and of high sensitive ones were 13 2 It is concluded that it is a quick mothed to use POX isoenzyme of millet shoots to identify the resistance to kernel smut of millet.