同工酶差异位点分析在蔬菜杂交种纯度检测中的应用

Determining Vegetable Hybrid Seed Purity by Isoenzyme Diagnostic Loci

用 10种同工酶和蛋白质分析体系 ,分析了 7种重要蔬菜的 71个杂交种与其亲本之间的差异位点 ,以及这些差异位点用于杂交种纯度检测的可能性和存在的问题。试验表明 ,作物的不同种类 ,杂交种与亲本之间的亲缘关系以及作物种类的遗传多态性 ,都会影响同工酶差异位点产生的多寡 ,从而影响到这一技术是否能用于此种作物的纯度检测。

Ten isoenzymes and seed proteins were analyzed in parental inbreds and corresponding F 1 hybrid seeds of 71 commercial varieties including Chinese cabbage,cauliflower,broccoli,tomato,sweet pepper,cucumber and water melon to determine seed purity.The results showed the species with high polymorphism isoenzyme banding pattern have more diagnostic loci between hybrid and their parental inbreds.Ten isoenzymes appeared different polymorphism.POX,PGM and EST phenotypes at 8 genetic loci were different between F 1 hybrids and their parental inbreds of the four Brassica species,cucumber and sweet pepper.Hybrids of tomato could be identified at two MDH loci and 6-PGD.Genetic stability of the diagnostic loci was analyzed and the obvious variation during seed germination from one to ten days was found.A model was developed to estimate seed purity.