单抗夹心LAB-ELISA检测猪伪狂犬病病毒的研究

Study of Monoclonal Antibodies Sandwich LAB-ELISA Detecting Pseudorabies Virus

在伪狂犬病病毒种特异性单克隆抗体的基础上建立了检测该病毒抗原的单抗夹心LAB -ELISA方法。结果表明 ,该方法不与其他常见病原体产生交叉反应 ,抗原的最低检出含量为 8 9μg/mL。检测时最佳采样部位是猪脑及扁桃体。对人工感染兔、自然感染猪以及临床可疑病猪的检出率分别为 75% ,75%及 72 7% ,因此本方法是一种具有良好特异性及较好敏感性的诊断方法。

A sandwich LAB-ELISA was developed to detect Pseudorabies virus (PrV) on the basis of species specific monoclonal antibodies in this study.The results showed that there was no cross reactions between the PrV and other common pathogens.The minimum concentration of the antigen that could be detected with this assay was 8.9 μg/mL.The most suitable organ to get samples was brain or tonsil.The positive rates to artificially infected rabbits,naturally infected swine and clinical dubious swine were 75%,75% and 72.7%.Therefore this assay was a suitable method with good specificity and sensitivity in the diagnosis of Pseudorabies.