针刺对偏头痛模型大鼠动脉血流动力学相关参数的影响

Influence of acupuncture on hemodynamic parameters in rats with migraine

目的应用超声测量偏头痛大鼠颈总动脉血流动力学参数,观察针刺对大鼠颈总动脉血流动力学参数的影响,从血流动力学方面初步探讨针刺对硝酸甘油诱发偏头痛的治疗作用及作用机制。方法将24只SD大鼠随机分为空白组、模型组、必理通组、针刺组4组,每组各6只。对模型组、必理通组、针刺组进行造模,空白组和模型组不予治疗,必理通组予必理通灌胃治疗,针刺组予针刺治疗,应用彩色多普勒超声测量大鼠颈总动脉的直径、收缩期峰值流速(Vs)、舒张末期流速(Vd)、平均流速(Vm)、搏动指数(PI)、阻力指数(RI)及心率(HR),计算颈总动脉血流量、收缩期峰值流速/舒张末期流速(Vs/Vd),对各组颈总动脉血流动力学参数进行比较。结果与空白组比较,必理通组、针刺组双侧颈总动脉血流量均明显升高(P<0.01),模型组双侧颈总动脉血流量轻度升高(P<0.05)。与模型组比较,针刺组双侧颈总动脉血流量明显升高(P<0.01)。与必理通组比较,针刺组右侧颈总动脉血流量升高(P<0.01)。与空白组比较,模型组、必理通组、针刺组双侧颈总动脉PI数值升高(P<0.01)。与必理通组比较,针刺组右侧PI升高(P<0.01)。各组颈总动脉直径、Vs、Vd、Vm、RI、Vs/Vd及HR比较差异均无统计学意义(P>0.05)。结论针刺对硝酸甘油诱发偏头痛大鼠的颈总动脉血流量有明显改善作用,颈总动脉血流量、PI与偏头痛密切相关,可作为治疗偏头痛较好的参考指标。

Objective To observe the influence of acupuncture on hemodynamic parameters of common carotid artery in rats with migraine, and discuss the curative effect and mechanism of acupuncture in treatment of migraine induced by nitroglycerin from the aspect of hemodynamies. Methods SD rats （n=24） were randomly divided into blank group, model group, Panadol group and acupuncture group （each n=6）. The diameter of common carotid artery, Vs, Vd, Vm, PI, RI and HR were detected by using color Doppler ultrasound, and common carotid artery blood flow and Vs/Vd were calculated. The hemodynamic parameters of common carotid artery were comparedamong all groups. Results Compared with blank group, bilateral common carotid artery blood flow increased significantly in Panadol group and acupuncture group （P〈0.01） and increased mildly in model group （P〈0.05）. Compared with model group, bilateral common carotid artery blood flow increased significantly in acupuncture group （P〈0.01）. Compared with Panadol group, right common carotid artery blood flow increased （P〈0.01）. Compared with blank group, PI of bilateral common carotid arteries increased in model group, Panadol group and acupuncture group （P〈0.01）. Compared with Panadol group, PI of right common carotid artery increased in acupuncture group （P〈0.01）. The differences in diameter of common carotid artery, Vs, Vd, Vm, RI, Vs/Vd and HR had no statistical significance among all groups （P〉0.05）. Conclusion Acupuncture has significant improving effect on common carotid artery blood flow in rats with migraine induced by nitroglycerin. Common carotid artery blood flow and PI are closely related to migraine, and they can be taken as reference indexes in treatment of migraine.