毛细管区带电泳测定饲料添加剂中酪蛋白磷酸肽含量的方法研究

Study on the Determination of Casein Phosphopeptides in the Feed Additive by Capillary Zone Electrophoresis

利用毛细管区带电泳 (CZE)对饲料添加剂中酪蛋白磷酸肽 (CPP)样品进行分离 ,结果显示 ,用四硼酸钠缓冲溶液(2 0mmol/L ,pH 9.2 )及 5 0cm× 70 μmi.d .石英毛细管柱 ,在工作电压 30kV、检测波长 2 0 0nm、柱温 2 5℃的条件下 ,CPP得到较好的分离 ;同时 ,采用外标法对饲料添加剂样品中CPP各组分的含量进行了测定。

The ability of separation and content test of CPP in the feed additive by capillary zone electrophoresis were discussed.The suit electrophoresis conditions were Na 2B 4O 7 Buffer solution (20 mmol/L,pH 9.2),50 cm×70 μm i.d. capillary cartridge, and V=30 kV、T=25 ℃、UV -200 nm.