三河马6-磷酸葡萄糖脱氢酶及葡萄糖磷酸异构酶的多态性

Polymorphism of 6-Phosphogluconate Dehydrogenase and Glucose Phosphate Isomerase in Sanhe Horses

采用淀粉凝胶电脉及琼脂覆盖技术对三河马血液红细胞 6 -磷酸葡萄糖脱氢酶和葡萄糖磷酸异构入酶的电泳变异进行了检测。在 6 -磷酸葡萄糖脱氢酶位点发现 3个表现型 ,即FF、FS和SS ,在葡萄糖磷酸异构酶位点共发现 2个表现型 ,即Ⅱ ,FI。等位基因频率直接通过表型计算得出 :PGDF 为为 0 840 ,PGDS 为 0 16 0 ,而GPIF 为 0 140 ,GPII 为0 86 0。父权否定概率在 6 -PGD和GPI位点分别为 0 116 3和 0 10 5 9。

Red cell 6-phosphogluconate dehydrogenase(6-PGD) and glucosephosphate isomerase(GPI)in Sanhe horses were detected in starch gels using agar overlay technique.Three phenotypes FF,FS,SS were found in 6-PGD locus and two phenotypes Ⅱ,FI in GPI locus.Computation of allelic frequencies was done by direct counting from phenotypes:6-PGD(F0.840,S0.160);GPI(F0.140,I0.860).The probability of exclusion of 6-PGD and GPI were 0.1163 and 0.1059,respectively.