IFN-γ增强人脐带间充质干细胞的免疫抑制能力

IFN-γ Stimulation Enhances Immunosuppressive Capability of Human Umbilical Cord Mesenchymal Stem Cells

本研究旨在探讨IFN-γ对脐带间充质干细胞免疫抑制能力的影响。利用细胞因子刺激细胞表面受体的方法改变MSC的免疫抑制能力。用MSC与淋巴细胞共培养实验检测激活后MSC对淋巴细胞增殖抑制的变化。用荧光定量PCR实验和ELISA实验检测激活后MSC基因和蛋白的表达变化情况。结果表明:IFN-γ可以增强脐带MSC的免疫抑制能力,IFN-γ激活后的MSC能更有效的抑制淋巴细胞的增殖,上调MSC细胞内免疫抑制基因IDO1,COX2和HLA-G等的表达和可溶性免疫抑制蛋白HLA-G、KYN、IL-10、PGE2等的分泌,并在细胞共培养实验中将间充质干细胞的免疫抑制功能提高2-7倍。结论:IFN-γ可有效提高MSC的免疫抑制能力。

This study was objective to explore the effect of IFN-γ on immunosuppressive capability of mesenchymal stem cells （MSC） derived from umbilical cord. The immunomodulating capability of MSC was changed by stimulating cell surface receptors like Toll-like receptors （TLR）. The inhibition of T-lymphocyte proliferation by MSC was tested via cell co-cultures. Further RT-PCR and ELISA were performed to examine the expression changes in gene and protein level. The results showed that the IFN-γ could promote the immunosuppressive effect of umbilical cord derived MSC. IFN-γ-stimulated MSC could suppress the proliferation of T cells more effectively. IFN-γ stimulation up-regulated the expression of immunosuppressive genes like IDOl, COX2, HLA-G, and soluble suppressive proteins such as HLA-G, KYN, ILl0, PGE2 of MSC. And the immuno suppression capability of IFN-γ-stimulated MSC was 2 - 7 folds higher than control in MSC and lymphocyte co-culture tests. It is concluded that IFN-γ can effectively enhance the immunosupp-ressive capability of MSC.