白细胞介素-6与胃癌易感性的相关研究

Association between polymorphism in interleukin-6 and risk of gastric cancer

目的 评价IL-6 174G/C和IL-6 572G/C基因多态性与胃癌风险的相关关系,以及IL-6基因多态性与幽门螺杆菌（H.pylori）感染和吸烟的交互作用.方法 采用病例对照研究方法,纳入咸宁市中心医院消化内科2008年1月-2011年5月新发胃癌246例,非肿瘤患者274例作为对照组.采用PCR-RFLP方法测定IL-6 174G/C和IL-6 572G/C的基因分型.结果 研究发现携带IL-6 174CC基因型和C等位基因型胃癌发病风险显著增高,调整后的OR（95％ CI）分别为1.88（1.07 ～3.48）和1.56（1.14～2.52）.携带IL-6 174GC和CC基因型在H.pylori阳性感染者中和吸烟者中均能增加患胃癌的风险,IL-6 174G/C基因多态性与H.pylori感染有交互作用（P＜0.05）.研究未发现IL-6 572G/C基因多态性与胃癌发病风险有相关关系.结论 本研究结果表明IL-6基因多态性具有促进胃癌发生和发展的作用,IL-6可以作为胃癌遗传学的检测指标,用于检验胃癌易感个体的生物学指标.

Objective To assess the polymorphism in interleukin-6 and risk of gastric cancer,and the interaction of polymorphism in interleukin-6 with H.pylori infection and smoking.Methods A total of 246 cases of gastric cancer were collected from Jan.2008 to May.2011,and 274 cancer-free cases were collected as controls.PCR-RFLP was taken to analyze the polymorphisms of IL-6 174G/C and IL-6 572G/C.Results H.pylori infection and smoking were the risk factors of gastric cancer.Individuals carrying IL-6 174CC and IL-6 174C allele could increase the risk of gastric cancer,with the OR （95％ CI） of 1.88 （1.07 ～3.48） and 1.56 （1.14 ～2.52）,respectively.Individuals carrying IL-6 174GC and CC genotypes could increase the risk of gastric cancer among those with positive H.pylori infection and smoking.Interaction between polymorphism in IL-6 174G/C and H.pylori infection was found.However,significant association of IL-6 572G/C polymorphism with risk of gastric cancer was not found.Conclusion Polymorphism in IL-6 174G/C could develop the gastric cancer.IL-6 174G/C polymorphism could be used as a biomarker for detection of gastric cancer.