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Subcellular localization of chitosan oligosaccharides in living cells 被引量:1

Subcellular localization of chitosan oligosaccharides in living cells
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摘要 Chitosan oligosaccharides(COS)is derived from the chemical or enzymatic decomposition of chitosan.The exact mechanisms underlying many biological functions of COS have not been elucidated.Since subcellular localization is very important in determining biological activity in a number of molecules,we used fluorescein isothiocyanate-labeled chitosan oligosaccharides(FITCCOS)and investigated the localization of COS in living cells by laser scanning microscopy.We found that COS entered cells in a dose-and time-dependent manner,and we first showed that COS may enter cells by facilitated passive diffusion and was preferentially localized in the mitochondria.While in high concentration,it was also found in the cytoplasm and nucleus and was enriched in the nucleolus and karyotheca.The different subcellular localization of COS suggested that they played different effects.Determination of COS subcellular localization in cells is important to help us understand the potent mechanisms underlying its multiple functions. Chitosan oligosaccharides (COS) is derived from the chemical or enzymatic decomposition of chitosan. The exact mechanisms underlying many biological functions of COS have not been elucidated. Since subcellular localization is very important in determining biological activity in a number of molecules, we used fluorescein isothiocyanate-labeled chitosan oligosaccharides (FITC- COS) and investigated the localization of COS in living cells by laser scanning microscopy. We found that COS entered cells in a dose- and time-dependent manner, and we first showed that COS may enter cells by facilitated passive diffusion and was preferentially localized in the mitochondria. While in high concentration, it was also found in the cytoplasm and nucleus and was enriched in the nucleolus and karyotheca. The different subcellular localization of COS suggested that they played different effects. Determination of COS subcellular localization in cells is important to help us understand the potent mechanisms underlying its multiple functions.
出处 《Chinese Science Bulletin》 SCIE EI CAS 2014年第20期2449-2454,共6页
基金 supported by the National Natural Science Foundation of China (81171459, 51072055, 51202069) Research Fund for the Doctoral Program of Higher Education of China (20110172110002) the Fundamental Research Funds for the Central University (2014ZM0009, 2012ZP0001, 2013ZM0043)
关键词 亚细胞定位 活细胞 壳聚糖 寡糖 脱乙酰壳多糖 激光扫描显微镜 COS 生物学功能 Biomaterials Chitosan oligosaccharides FITC Subcellular localization Mitochondrion
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