摘要
Cell membranes play a key role in cellular activities.Fish erythrocytes,prototype of the nucleated erythrocytes of lower vertebrates,are predominantly oval,biconvex discs with elliptical nucleus and much larger in size than human erythrocytes.In attempts to disclose the correlation of membrane structure between fish and human erythrocytes,we used in situ atomic force microscope(AFM)combined with single-molecule force spectroscopy to study the membrane structure of Crucian carp erythrocytes under quasi-native conditions.Our results revealed the asymmetric distribution of proteins in Crucian carp erythrocyte membrane:the outer leaflet of membrane is rather smooth without any proteins,whereas the inner leaflet of membrane is very rough with dense proteins.The asymmetry of fish erythrocyte membrane structure fits well with the semi-mosaic model of human erythrocyte membrane structure.This similarity of membrane structure between human and fish erythrocytes extends the semimosaic model of erythrocytes membrane structure to a wider range of species.
Cell membranes play a key role in cellular activities. Fish erythrocytes, prototype of the nucleated erythrocytes of lower vertebrates, are predominantly oval, biconvex discs with elliptical nucleus and much larger in size than human erythrocytes. In attempts to disclose the correlation of membrane structure between fish and human erythrocytes, we used in situ atomic force microscope （AFM） combined with single-molecule force spectroscopy to study the membrane structure of Crucian carp erythrocytes under quasi-native conditions. Our results revealed the asymmetric distribution of proteins in Crucian carp erythrocyte membrane： the outer leaflet of membrane is rather smooth without any proteins, whereas the inner leaflet of membrane is very rough with dense proteins. The asymmetry of fish erythrocyte membrane structure fits well with the semi-mosaic model of human erythrocyte membrane structure. This similarity of membrane structure between human and fish erythrocytes extends the semimosaic model of erythrocytes membrane structure to a wider range of species.
基金
supported by Ministry of Science and Technology of China (2011CB933600)
the National Natural Science Foundation of China (21373200,31330082,21203177)
