高交感活性诱导的大鼠心肌损伤模型中氧化应激的受体调控机制

Dependence of adrenoceptor regulation on oxidative stress in cardiac injury induced by high sympathetic activity in rats

目的:研究高交感活性诱发大鼠心肌损伤的氧化应激受体调控机制。方法:Sprague-Dawley(SD)大鼠随机分为对照组、模型组、普萘洛尔(Pro)组、哌唑嗪(Praz)组、普萘洛尔+哌唑嗪(Pro+Praz)组、维生素E(VE)组及普萘洛尔+哌唑嗪+维生素E(Pro+Praz+VE)组,除对照组外其余各组均腹腔注射去甲肾上腺素(NE)复制高交感活性引起的心肌损伤模型,同时灌胃给予相应药物,连续给药16 d,期间监测各组动物体重的变化。16 d后进行心室重构指标(心指数和羟脯氨酸含量)、病理组织学检查、氧化/抗氧化指标(MDA、SOD、CAT、GSH-Px和T-AOC)和能量代谢指标(Na+-K+ATPase和Ca2+-Mg2+ATPase)分析。结果:从第9天开始,模型组动物体重与对照组的比较差异有统计学意义(P<0.05),心指数和左心室肥厚明显增加,氧化/抗氧化和能量代谢障碍;Pro、Praz、Pro+Praz和VE各组均出现不同程度的动物体重、心指数、左心室肥厚和氧化/抗氧化失衡的改善;Pro、Praz和Pro+Praz能明显升高左心室Na+-K+ATPase和Ca2+-Mg2+ATPase的活性,Pro+Praz作用最明显(P<0.05)。结论:肾上腺素受体依赖是高交感活性诱导心肌氧化应激损伤的重要途径。

AIM：To investigate the dependence of the adrenoceptor regulation on oxidative stress in the rats with cardiac injury induced by high sympathetic activity .METHODS： Healthy SD rats were randomly divided into 7 groups：control, model, propranolol （Pro）, prazosin （Praz）, Pro＋Praz, vitamin E （VE） and Pro＋Praz＋VE.The rats were intraperitoneally injected with norepinephrine （ NE） for continuous 16 d to reproduce cardiac injury , and treated with the respective drugs .During the experimental process , the body weight was recorded .At the end of the experiments , the following parameters were measured：the ventricular remodeling indexes （ cardiac index and hydroxyproline of the left ventricle）, histopathologic examination , oxidative/antioxidative indexes [MDA, SOD, catalase （CAT）, GSH-Px and total an-tioxidant capacity （T-AOC）], and energy metabolism （Na^＋-K^＋ATPase and Ca^2＋-Mg^2＋ATPase）.RESULTS： The in-crease of body weight in model group was significantly slower than that in control group after 9 d of treatment （P〈0.05）. The cardiac index and left ventricular hypertrophy were significantly increased .Oxidation/antioxidation and energy metabo-lism were disturbed.In Pro, Praz, Pro＋Praz and VE groups, the body weight, cardiac index, left ventricular fibrosis and oxidative/antioxidative dysfunction were ameliorated .Pro, Praz and Pro ＋Praz increased the activity of Na ^＋-K^＋ATPase and Ca^2＋-Mg^2＋ATPase.Treatment with Pro＋Praz showed the best result in all of the indexes （P〈0.05）.CONCLU-SION：The dependence of adrenoceptor regulation plays an important role in the formation of oxidative stress in the process＆amp;nbsp;of rat cardiac injury induced by high sympathetic activity .