大白猪MUC13基因多态性与生长、肉质性状的相关性

The polymorphism of MUC13 gene of large white pig and its correlation with the traits of growth and meat quality

选择对仔猪腹泻有显著影响的产肠毒素大肠杆菌(ETEC)F4ac的受体基因MUC13为研究对象,采用PCR-SNaPshot判型与常规育种技术相结合的方法,对544头大白猪核心育种群进行基因判定及基因型与生长、肉质性状相关性的研究.结果表明,大白猪MUC13基因型分布大小为:腹泻易感杂合基因型GA>腹泻抗性纯合基因型GG>腹泻易感纯合基因型AA,腹泻抗性有利基因G的等位基因频率为0.669,腹泻易感不利基因A的等位基因频率为0.331.3种基因型个体达100kg日龄及其估计育种值(EBV)、达100 kg背膘厚及其EBV的大小为:AA>GA>GG;眼肌面积、肌内脂肪含量和估计瘦肉率的大小为:GG>GA>AA,差异不显著(P>0.05).可见,在种猪选育上,逐代选择带有G等位基因的个体,建立抗性纯合GG基因型个体的种猪核心群,即可降低仔猪腹泻率,又不影响生长和肉质的性状.

With 544 nucleus breeding large white pigs as material, PCR-SNaPshot and conventional breeding test was used to deter-mine the genotypes, and the correlations between genotypes of MUCl3 and the traits of growth and meat quality were analyzed. Theresult showed that the frequencies of 3 genotypes were GA 〉 GG 〉 A.A, G allele frequency was 0.669, A allele frequency was 0.331.The ages of 100 kg body weight and its estimated breeding value （EBV） of the 3 genotypes were AA 〉 GA 〉 GG, as well as the backfat thickness of 100 kg body weight and its EBV. Eye muscle area, intramuscular fat content, and estimated lean meat percentage ofthe 3 genotypes were GG 〉GA 〉AA , which were not significant （P 〉0.05）. Therefore, in the selection process of pig breeding, theindividuah with allele G should be selected generation-by-generation for reducing diarrhea rate of piglets, establishing a favorable gen-otype of MUCl3 pure line core group, which would not affect the growth rate and meat quality traits in the breeding large white pigs.