响应面法优化黑水虻幼虫蛋白质提取工艺

Optimization of extraction of proteins from larvae of the black soldier fly, Hermetia illucens( Diptera:Stratiomyidae ), using response surface methodology

【目的】对黑水虻Hermetia illucens幼虫蛋白质进行不同提取方法的比较,选择最优提取方法,并确定其最优工艺参数,为黑水虻幼虫蛋白提取与资源利用提供依据。【方法】以黑水虻幼虫为原料,分别采用碱提法、酶提法、盐提法和Tris-HCl缓冲液提法对黑水虻幼虫蛋白质进行提取,并比较分析。通过单因素试验分别确定NaOH质量浓度、液料比、提取温度及提取时间4个因素的较优水平。在单因素试验结果基础上,按照Box-Behnken响应面试验设计进行响应面优化试验。【结果】提取黑水虻幼虫蛋白质的4种方法中碱提法的提取率最高,最佳提取条件为:NaOH质量浓度2.44 g/100mL,液料比22 mL/g,提取温度53℃,提取时间2 h。提取率验证试验结果为88.49%,与预测值相对误差为0.28%。【结论】响应面模型拟合度高,优化出的最佳提取工艺可行。

[ Aim ] To compare four different extraction methods of proteins from larvae of the black soldier fly, Hermetia illucens, choose the method with highest extraction rate, and obtain the optimum process parameters so as to provide reference to protein extraction and resource utilization of It. illucens. [ Methods ] The methods of extraction by lye, enzyme, salt and Tris-HCl buffer were used and comparatively analyzed in extracting proteins from larvae of the black soldier fly. Then, the optimum levels of NaOH mass concentration, liquid-to-solid ratio, extraction temperature and time were determined by means of single factor tests. The experiment and analysis of Box-Behnken response surface was conducted based on the results of univariate tests and the actual situation. [ Results ] Among the four extraction methods of proteins from black soldier fly larvae, lye extraction was optimal, with the optimum extraction conditions of NaOH mass concentration 2. 44 g/100 mL, liquid-to-solid ratio 22 mL/g, extraction temperature 53 ℃ and extraction time 2 h. The corresponding extraction ratio of verification test was 88.49%, with the relative error of only 0.28% compared with predictive value. [ Conclusion] The response surface model is good fit, and the optimum extraction process is feasible.