polyCoTAPC/GOD/Nafion生物传感器的研究及其对体液中葡萄糖含量的测定

Liquid Chromatography-Electrochemical Detector for the Determination of Clucose in microdialysis of Rat Brain in Vivo

本文研制了一种高效、灵敏的葡萄糖生物传感器。通过在玻碳 (GC)电极表面先后修饰四胺基钴酞菁 (CoTAPC)和葡萄糖氧化酶 (GOD) ,最后涂敷一层Nafion膜 ,获得了抗干扰力强、灵敏度高、稳定性好、寿命长的葡萄糖生物传感器 ,在 1 0× 10 -5 ～ 5 0× 10 -3 mol/L浓度范围内与峰电流呈良好的线性关系 ,寿命达一个月以上。同时 ,本文将微渗析取样技术与HPLC -电化学检测 (HPLC -EC)联用测定了大鼠脑皮层中的葡萄糖(glucose)含量 ,结果为 0 74± 0 0 1mmol/L (n=4 )。

in this paper,microdialysis sampling was combined with liquid chromatography with electrochemical detection(LC-ECD)and determined glucose in rat brain.A CoTAPC/ glucose oxidase(GOD)/Nafion film-coated glassy carbon electrode was used to selectively detect the hydrogen peroxide generated enzymatically in the enzyme reactors,withour any interference from electroactive species such as L-ascorbate,urate,proteins.The signal current was linearly related to the glucose concentration in the range of 1 0×10 -6 ～5 0×10 -3 mol/L at+0 5V(vs.Ag/AgCl) with a detection limit of 5 0×10 -7 mol/L.To measure the glucose in rat brain,microdialysis sampling was employed.The dialysate from the microdialysis system was injected into system.The result was 0 7±0 10mmol/L (n=4)。