用于高密度多焦点多光子显微成像系统的时间延板设计

Design of Time-Delaying Plate Used in High-Density Multifocal Multiphoton Microscopic Imaging System

多焦点多光子显微(MMM)和单点扫描多光子显微相比,可显著提高光能利用率和成像速度,在生命科学研究领域具有广泛的应用前景。但现有MMM技术成像质量和成像速度之间存在矛盾,增加单位面积内的焦点数目可提高成像速度,但过小的焦点间距离会产生子光束串扰,从而影响纵向空间分辨率。利用Zemax软件模拟产生了高密度激发点阵,将传统的不小于6 mm的相邻焦点间距缩小到约3 mm;分析了高密度点阵激发成像产生噪声的原因;根据时间复用技术的原理设计正方形时间延板,对相邻子光束脉冲进行不同时间的延迟,从而可以消除MMM系统中高密度激发点阵子光束间的串扰。

Compared with single- point scanning microscopy, multifocal multiphoton microscopy （MMM） can significantly improve the light energy utilization efficiency and imaging speed. Thus it has broad application prospects in life science. Unfortunately, there exists conflicts between the image quality and imaging speed in the available MMM systems. Although increasing focus number within a unit area can increase the imaging speed, too small focus distance will produce crosstalk between adjacent beamlets that will affect the longitudinal spatial resolution. High- density excitation focus array is simulated with Zemax, the distances between adjacent focus are reduced from 6 mm to 3 mm; the causes of noise in high- density multifocal multiphoton microscopic imaging system are analyzed; a square time- delaying plate based on the principle of time multiplexing technology is designed, which can generate different time delays between adjacent subpulses, therefore the crosstalk between adjacent beamlets is eliminated in high-density MMM imaging system.