野生大豆回交导入系蛋白质含量性状的QTL分析

Genotyping and QTL mapping of protein content with wild soybean backcross introgressive lines

以野生型大豆ZYD00006(供体亲本)与黑龙江省主栽品种绥农14(轮回亲本)所构建的回交导入系(1 204株)为研究材料,利用WinQTL2.5的复合区间作图法(CIM)在9个连锁群定位了16个与蛋白质含量相关的QTL(14个正效应,2个负效应);导入系群体经过严格的蛋白质含量筛选鉴定,得到10个蛋白质含量性状明显大于轮回亲本的导入系株行。利用这10个高蛋白含量株行(选择群体)结合随机对照群体,通过基于遗传搭车原理的卡方分析,检测到分布于10个连锁群上的17个与大豆蛋白质含量相关的标记位点,对蛋白质含量表现为正效应。两种方法共同检测到7个QTL。这些材料和位点将为高蛋白含量相关基因克隆及分子辅助育种提供重要的材料基础和标记信息。

A set of backcross population were constructed with Suinong 14 as recurrent parent and wild soybean ZYD00006 as donor parent. 16 QTLs （ 14 QTLs with positive effect, 2 QTLs with negative effect） for protein content among 9 linkage groups detected by composite interval mapping （CIM） with Win QTL2.5. Ten BC3F3 lines with high p as control （ rotein content compared with Suinong 14 were screened out, and another 10 lines from BC3 F3 were used random population）. QTLs for protein content on 10 linkage groups were detected with genotype analysis by chi - square on genetic hitchhiking, and all showed positive effect. 7 QTLs were detected by both methods. These materials and markers provided important material foundation and marker information for gene cloning and molecular assisted breeding in high protein content.