离子液体双水相萃取测定卷烟烟气中酚类物质的研究

Two aqueous phase extraction of ionic liquid combined high performance liquid chromatographic for the determination of phenols in cigarettes stream

建立了卷烟烟气中邻-苯二酚、间-苯二酚、对-苯二酚、苯酚、邻-甲酚、间-甲酚和对-甲酚几种酚类物质的高效液相色谱检测方法.用25%的乙醇水溶液对卷烟样品进行超声提取,再经离子液体双水相分离富集,用高效液相色谱二极管阵列检测器(HPLC-FLD)测定.该方法的富集倍数可以达到20倍,整个操作过程在常温下进行,时间不超过15 min.实验结果表明,在0.02～20 ng/L范围内,几种酚类物质的峰面积均与其质量浓度呈线性相关,R2≥0.999 6,检出限分别为0.06,5.2,1.2,5.3,0.03,0.04 ng/L,相对标准偏差为1.4%～4.6%,回收率为85.1%～96.9%.该方法具有良好的重现性、精密度和更低的检测限.

A method was established by high - performance liquid chromatography （ HPLC - FLD） for deter- mination of catechol,resorcinol, hydroquinone, phenol, o - cresol and （p ＋ m） - cresol in flavor. The samples were enriched by two aqueous phase extraction （TAPS） of ionic liquid and then detected by high - performance liquid chromatography - fluorescence detector （ HPLC - FLD）. The enrichment factor of the method up to 20 and the whole operation process less than 15 min. In the range of 0.02--20 ng/mL, there was a good correlation （R^2 ≥0. 999 6） between the peak area and concentration of catechol,resorcinol, hydroquinone, phenol,o - cresol and （p ＋ m） - cresol. The detection limits of phenols were 0. 06 ,5. 2 ,1. 2 ,5. 3 ,0. 03 ng/L and 0.04 ng/L respectively. The relative standard deviations（RSD） were 1.4%-4.6% and the recoveries were 85.1%-96.9%. Compared with the previous methods, this method has good reproducibility, precision and lower detection limit.