ERa基因多态性与月经过少相关性研究

Correlation between gene polymorphism of estrogen receptor-a and hypomenorrhea

目的探讨雌激素受体a(ERa)基因PvuⅡ、XbaⅠ基因多态性在月经过少人群中的分布情况。方法采用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)分析方法检测实验组及正常组人群的外周血ERa基因多态性,比较2组的PvuⅡ、XbaⅠ基因型、等位基因计数的分布差异。结果 ERa PvuⅡ基因型PP、Pp、PP及XbaI基因型XX、Xx、xx在实验组和正常组的频率分布均有显著性差异(P<0.05),实验组基因型的构成比依次为:PP(5.45%)、Pp(40%)、pp(54.55%),XX(1.82%)、Xx(36.36%)、xx(61.82%);正常组基因型的构成比依次为:PP(18.18%)、Pp(41.91%)、pp(41.91%),XX(9.09%)、Xx(50.00%)、xx(40.91%)。P、p和X、x等位基因频率在实验组和正常组的频率分布有显著性差异(P<0.05)。P等位基因患月经过少的风险是p等位基因的0.55倍;X等位基因患月经过少的风险是x等位基因的0.483倍。结论 ERa基因多态性PvuⅡ和XbaI与月经过少存在相关性,p和x等位基因可能是其危险因素;pp基因型和xx基因型可能是其易感基因多态性。

Objective To investigate the distribution of gene polymorphism of PvuII and XbaI in gene 1 of estrogen receptor-a （ERa） in patients with hypomenorrhea. Methods The gene polymorphism of ERa in peripheral blood was detected by using polymerase chain reaction-restriction fragment length polymorphism （PCR-RFLP） in experimental group and normal group. The distribution difference in genotypes of Pvu Ⅱ and Xba I and allele count were compared between two groups, Results The frequency distribution of genotypes of PP, Pp and pp of ERa PvuII and genotypes of XX, Xx and xx XbaI had significant difference between two groups （ P 〈 0.05 ）. The constituent ratio of genotypes in experimental group was orderly as follows ： PP ： 5.45 % , Pp ：40.00 % , pp ： 54.55 % , XX ： 1.82 % , Xx ： 36.36% ,and xx：61.82%. The constituent ratio of genotypes in normal group was orderly as follows： PP ： 18.18 % , Pp ： 40.91% , pp ： 40.91% , XX ： 9.09 % , Xx ： 50.00 % , and xx ： 40.91%. The frequency distribution of allele P, p and X, x had significant difference between two groups （ P 〈 0.05 ）. The risk of hypomenorrhea of allele P was 0.55 times higher than allele p, and allele X was 0. 483 times higher than allele x. Conclusion There is correlation between gene polymorphism of PvuⅡ and XbaI of Era and hypomenorrhea. Allele p and allele x may be the risk factors, and genotype pp and genotyme xxmay be susceptible to hypomenorrhea.