期刊文献+
任意字段
题名或关键词
题名
关键词
文摘
作者
第一作者
机构
刊名
分类号
参考文献
作者简介
基金资助
栏目信息

利用猪流行性腹泻病毒S基因真核表达质粒制备多克隆抗体 被引量：1

Preparation of polyclonal antibody with S gene eukaryotic expression plasmid of porcine epidemic diarrhea virus
下载PDF
导出
摘要 将流行性腹泻病毒(PEDV)S基因的真核表达质粒pVAX1-S作为抗原,免疫BALB/c小鼠制备多克隆抗体。抗体经纯化后,利用间接ELISA、间接免疫荧光、Western Blot方法检测制备多克隆抗体的生物学活性。结果表明,用PEDV S基因真核表达质粒免疫制备的多抗血清抗体效价可达1??214,该多克隆抗体不仅可以很好地识别PEDV S蛋白,还可以特异性检测到PEDV抗原。研究为PEDV的诊断和S蛋白免疫学特性研究提供试验依据。 This experiment was undertaken to prepare polyclonal antibody by immunization of BALB/c mouse with PEDV S gene eukaryotic expression plasmid （pVAX1-S） as antigen. The biological activity of the polyclonal antibody was assayed by indirect ELISA, indirect immunofluorescence （IF） test and Western Blot. The results showed that the antiserum titer was 1∶214. Not only the recombinant S protein could be identified by the polyclonal antibody, but also the PEDV-derived S protein. This study provided experimental basis of characterization of PEDV immunological properties and disease diagnosis.
作者 任晓峰 王雪 高睿泽 董博 李广兴
机构地区 东北农业大学动物医学学院
出处 《东北农业大学学报》 CAS CSCD 北大核心 2014年第6期61-66,共6页 Journal of Northeast Agricultural University
基金 国家自然科学基金面上项目(31372438) 教育部博士点基金项目(20122325110019) 黑龙江省普通高等学校长江学者后备支持计划项目(2013CJHB002)
关键词 猪流行性腹泻病毒 S蛋白 真核表达质粒 多克隆抗体 porcine epidemic diarrhea virus S gene eukaryotic expression plasmid polyclonal antibody
分类号 S828 [农业科学—畜牧学] S851 [农业科学—预防兽医学]
  • 相关文献

参考文献13

  • 1Bridgen A, Duarte M, Tobler K, et al. Sequence determination of the nucleocapsid protein gene of the porcine epidemic diarrhoea virus confirms that this virus is a eoronavirus related to human coronavirus 229E and porcine transmissible gastroenteritis virus [J]. Gen Viro I, 1993, 174: 1795-1804.
  • 2Duarte M, Tobler K, Bridgen A, et al. Sequence analysis of the porcine epidemic diarrhea virus genome between the nucleocap- sid and spike protein genes reveals a polymorphic ORF[J]. Virology, 1984, 198: 466-476.
  • 3DeBouck P, Callebaut P, Pensaert M. Prevalence of the porcine epidemic diarrhea (PED) virus in the pig population of different countries[J]. Vet Soc, 1982(7): 53.
  • 4Hofmann M, Wyler R. Serologic study of the occurrence of epizoot- ic viral diarrhea in swine in Switzerland[J]. Schweiz Arch Tierhe- ilkd, 1987, 129: 437.
  • 5Mostl K, Horvath E, Burki F. Erhebungen uber porcine coronavi-ten in osterreich II porcine epidemic diarrhea virus(PEDV) der schweine[J], Wien Tierarztl Monatssch, 1990, 77: 10-18.
  • 6Jimene Z G, Castro J M, Pozzo M, et al. Identification of a corona- virus inducing porcine gastroenteritis in Spain[J]. Pig Vet Soc Congr, 1985(9): 186.
  • 7Kweon C H, Kwon B J, Jung T S, et al. Isolation of porcine epi- demic diarrhea virus (PEDV) in Korea[J]. Korean Vet Res, 1993, 33: 249-254.
  • 8Pensaert M B, de Bouck P. A new coronavirus-like particle asso- ciated with diarrhea in swine [J]. Arch Virol, 1978, 58: 243-247.
  • 9Matthijnssens J, Ciarlet M, Heiman E, et al. Full genome-based classification of rotavimses reveals a common origin between human Wa-Like and porcine rotavirus strains and human DS-1- like and bovine rotavirus strains[J]. J Virol, 2008, 82: 3204- 3219.
  • 10孙东波,冯力,时洪艳,陈建飞,崔晓辰,佟有恩.猪流行性腹泻病毒S蛋白中和表位区单克隆抗体的制备与鉴定[J].中国预防兽医学报,2007,29(11):887-890. 被引量：13

二级参考文献9

  • 1郭慧芳,张文红,温冬青,韩锋产,张虎明,罗进,阎小君.UreB蛋白B细胞抗原表位快速筛选与鉴定[J].生物化学与生物物理进展,2006,33(1):83-86. 被引量：4
  • 2郭东华,徐世文,李术.阿维菌素单克隆抗体的制备与鉴定[J].中国兽医科学,2006,36(12):1019-1023. 被引量：3
  • 3Pensaert M B, Debouck P. A new coronavims-like particle associated with diarrhea in swine [J]. Arch Virol, 1978, 58(3): 243-247.
  • 4Shibata I, Tsuda T, Moil M, et al. Isolation of porcine epidemic diarrhea virus in porcine cell cultures and experimental infection of pigs of different ages [J]. Vet Microbiol, 2000, 72(3-4): 173-182.
  • 5Knipe D M, Howley P M, Griffin D E, et al. Coronaviruses in Fields Virology [M]. 4th ed. Philadelphia: Lippincott Williams & Wilkins, 2001.
  • 6Kang T J, Seo J E, Kim D H, et al. Cloning and sequence analysis of the Korean strain of spike gene of porcine epidemic diarrhea virus and expression of its neutralizing epitope in plants [J]. Protein Expr Purif, 2005, 41(2): 378-383.
  • 7Cruz D J, Kim C J, Shin H J, et al. Phage-displayed peptides having antigenic similarities with porcine epidemic diarrhea virus (PEDV) neutralizing epitopes [J]. Virology, 2006, 354(1): 28-34.
  • 8Krempl C, Herrler G. Sialic Acid Binding Activity of Transmissible Gastroenteritis Coronavirus Affects Sedimentation Behavior of Virions and Solubilized Glycoproteins [J]. J Virol, 2001, 75 (2): 844-849.
  • 9戴和平,高宏,赵新颜.噬菌体展示技术的原理和方法[J].水生生物学报,2002,26(4):400-409. 被引量：11

共引文献12

同被引文献17

  • 1Coll J M.The glycoprotein G of rhabdoviruses[J].Arch Virol,1995,140(5):827-851.
  • 2Grinnell B W,Wagner R R.Nucleotide sequence and secondary structure of VSV leader RNA and homologous DNA involved in inhibition of DNA-dependent transcription[J].Cell,1984,36(2):533-543.
  • 3Petersen J M,Her L S,Varvel V.The matrix protein of vesicular stomatit is virus inhibits nucleocytoplasmic transport when it is in the nucleus and associated with nuclear pore comp lexes[J].Mol Cell Biol,2000,20:8590-8601.
  • 4Reiss C S,Plakhov I V,Komatsu T.Virus replication in olfactory receptor neurons and entry into the olfactory bulb and brain[J].Ann N Y Acad Sci,1998,855:751-761.
  • 5MiyoshK,Harter D H,Hsu K C.Neuropathological and immunofluo rescence studies of experimental vesicular stomatitis virus encephalitis in mice[J].Neuropathol Exp Neurol,1971,30:266-277.
  • 6Hu J,Mu C,Hao J.Cerebral ischemia reduces expression of Hs1-associated protein X-1(Hax-1)in mouse brain[J].Neurosci Lett,2013,534:338-343.
  • 7Stojdl D F,Lichty B D,TenOever B R,et al.VSV strains with defects in their ability to shutdown innate immunity are potent systemic anti-cancer agents[J].Cancer Cell,2003,4(4):263-275.
  • 8Sinon K O,Whitaker-Dowling P A,Younger J S.Sequen tia disassembly of the cytoskeleton in BHK21cells in fected with vesicular stomatitis virus[J].Virology,1990,177(1):289-297.
  • 9Melki R,Gaudin Y,Blondel D.Interaction between tubulin and the viralmatrix protein of vesicular stomatitis virus possible in plrcation in the viral cytopathic effect[J].Virology,1994,202(1):339-347.
  • 10Cervera M,Dreyfuss G,Penman S.Messenger RNA is translated when associated with the cytoskeletal framework in normal and VSV-infected Hela cells[J].Cell,1981,23(1):113-120.

引证文献1

东北农业大学学报
2014年 第6期

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部