摘要
目的:研究还原型谷胱甘肽(GSH)对内毒素致大鼠心肌损伤的保护作用及其机制。方法:Wistar大鼠24只,随机分成对照组,内毒素组,内毒素+GSH组,每组8只。内毒素组:腹腔注射LPS;内毒素+GSH组:腹腔注射LPS和GSH;对照组:注射等剂量生理盐水。用HE染色法观察大鼠心肌组织的病理学改变,ELISA法检测血清及心肌组织匀浆中的cTnI、内皮素-1的含量结果:光镜下观察内毒素组心肌组织损伤严重,内毒素+GSH组心肌组织损伤程度较正常对照组重,但较内毒素组轻;与正常对照组比较,内毒素组血清及心肌组织匀浆中CTnI、ET-1显著升高(P<0.01),内毒素+GSH组血清及心肌组织匀浆中CTnI、ET-1较正常对照组有所升高,但较内毒素组低(P<0.05)。结论:内皮素-1介导心肌血管收缩可能参与了内毒素致大鼠心肌损伤的发生;GSH可以减轻内毒素致大鼠的心肌损伤,可能与减少ET-1生成有关。
Objective ： To observe the myocardial injury in rats with endotoxemia, and the intervention effect of GSH and its mechanisms of protection on septic myocardial injury. Methods ： Twenty-four Wistar rats were randomly divided into three groups, and eight in each： LPS group： intraperitoneal injection of LPS; LPS ＋ GSH group ： intraperitoneal injection of LPS and; normal control group （NS group）： intraperitoneal injection of the same dose of NS. Take part of heart HE dyeing to observe the change of myocardial use microscope. Detect the serum cTnI and ET-1 by ELISA; Results： （1）LPS group has severe myocardial injury observed under light microscope, LPS ＋ GSH group myocardial tissue injury more severe than Normal control group, but lighter than LPS group. （2） Compared with normal control group, serum levels of cTnI and ET-1 in the LPS group were significantly higher （P〈0.01）, serum of cTnI and ET-1 in LPS ＋ GSH group were higher than the normal control group, but lower than the LPS group （P 〈0.05）. Conclusion： Endothelin myocardial vasoconstriction involved in the pathogenesis of myocardial injury in endotoxemia. GSH can relieve the myocardial injury in rats with endotoxemia, probably through reducing the ET-1 on the expression.
出处
《内蒙古中医药》
2014年第15期114-117,共4页
Inner Mongolia Journal of Traditional Chinese Medicine
