赖氨酰氧化酶样-4与尿激酶型纤溶酶原激活物及其受体在人宫颈癌细胞中的表达及意义

Expressions and significance of lysyloxidase-like 4 and uPA/uPAR in human cervical carcinoma cell lines

目的探讨赖氨酰氧化酶样基因-4(LOXL4)、尿激酶型纤溶酶原激活物(uPA)及其受体(uPAR)在人宫颈癌细胞株中的表达。方法采用半定量RT-PCR方法检测人宫颈癌细胞株Hela、ME180、HCC94中LOXL4、uPA和uPAR mRNA的表达,同时运用Western blot技术检测各细胞中LOXL4蛋白的翻译情况。结果 LOXL4mRNA在三株人宫颈癌细胞株中表达差异有统计学意义(P<0.01),其中在Hela细胞株中表达浓度最高,ME180细胞株表达浓度次之,而HCC94细胞株表达浓度最低;而uPA、uPAR mRNA表达浓度水平在HCC94细胞中最高,明显高于Hela、ME180细胞的表达浓度水平(P<0.05)。结论 LOXL4、uPA、uPAR基因表达可能与宫颈癌细胞的侵袭和迁移有关。

Objective To investigate the expression of lysyloxidase-like 4（LOXL4）,urokinase plasminogen activator（uPA）,and urokinase plasminogen activator receptor（uPAR）in human cervical carcinoma cell lines.Methods LOXL4,uPA and uPAR mRNA expression levels in cell lines Hela,ME180,and HCC94were investigated by the method of reverse transcription PCR（RT-PCR）,determination of LOXL4protein levels in cell lines Hela,ME180,and HCC94with western blot.Results Differential expressions were found both LOXL4mRNA and protein levels of all the three human cervical carcinoma cells（P〈0.01）.Hela had the highest expression,and was followed by ME180and HCC94,respectively.The expression levels of uPA and uPAR mRNA were highest in HCC94cell lines,and significantly higher than that of Hela and ME180cell lines（P〈0.05）.Conclusion The expression of LOXL4mRNA,uPA and uPAR mRNA in human cervical carcinoma cell lines might be associated with invasion and metastasis of cervical cancer cells.