微卫星多态性和多位点序列分析技术在光滑念珠菌基因分型中的应用评估

Application evaluation of microsatellite polymorphism and multilocus sequence typing in genotyping of Candida glabrata

目的评估微卫星多态性分析技术在临床光滑念珠菌基因分型中的作用。方法收集2011年1月至2012年12月仁济医院和东方医院临床分离的光滑念珠菌59株,采用多位点序列分析(MLST)和微卫星多态性分析技术进行基因分型,比较2种基因分型方法的结果和分辨力。结果 59株光滑念珠菌经MLST分型得到6个序列型,其中ST-7型43株、ST-10型7株、ST-15型和ST-55型各3株、ST-3型2株、ST-43型1株,鉴别力指数(DP)为0.456;经微卫星多态性分析方法分为10型,其中A型25株、B型10株、C型8株、D型6株、E型3株、F型和G型各2株、H^J型各1株,DP为0.770。结论微卫星多态性分析方法简便、快速,分辨力高于MLST技术,可作为实验室基因分型的首选方法。

Objective To evaluate the application significance of microsatellite polymorphism in genotyping of Candida glabrata.Methods From January 201 1 to December 201 2,59 isolates of Candida glabrata were collected from Renji Hospital and East Hospital.All the 59 isolates were typed by microsatellite polymorphism and multilocus sequence typing （MLST）.The results and discrimination of the 2 genotyping methods were compared.Results By MLST,the 59 isolates belonged to 6 clone sequences,including 43 isolates with ST-7,7 isolates with ST-1 0,3 isolates with ST-1 5,3 isolates with ST-55,2 isolates with ST-3 and 1 isolate with ST-43,and the index of discriminatory power （DP）was 0.456.By microsatellite polymorphism,the 59 isolates were classified into 1 0 genotypes.There were 25 isolates with type A,1 0 isolates with type B,8 isolates with type C,6 isolates with type D,3 isolates with type E,2 isolates with type F,2 isolates with type G,1 isolate with type H,1 isolate with type I and 1 isolate with type J,and the DP was 0.770.Conclusions Microsatellite polymorphism is a simple and rapid method for molecular typing with higher discrimination than MLST.Therefore,microsatellite polymorphism can be the preferred choice in clinical laboratories.