人外周血单个核细胞miR-UL112水平在HCMV感染中的诊断价值研究

The diagnosis significance of miR-UL112 level in human peripheral blood mononuclear cells during HCMV infection

目的探讨单个核细胞中人巨细胞病毒(HCMV)微小RNA(miR)-UL112-3p、miR-UL112-5p水平对HCMV潜伏和激发感染的诊断价值。方法选取非肿瘤患者92例[其中HCMV特异性CD8+T淋巴细胞高水平组(简称CD8+细胞高水平组)57例、HCMV特异性CD8+T淋巴细胞低水平组(简称CD8+细胞低水平组)35例]、肿瘤化疗患者30例,提取外周血单个核细胞,采用实时荧光定量-聚合酶链反应(PCR)检测HCMV DNA、miR-UL112-3p及miR-UL112-5p水平。采用受试者工作特征(ROC)曲线评价miR-UL112-3p和miR-UL112-5p水平对HCMV潜伏感染的诊断价值。结果肿瘤化疗组HCMV DNA、miR-UL112-3p及miR-UL112-5p水平明显高于非肿瘤组(P<0.05)。非肿瘤组中CD8+细胞高水平组HCMV DNA、miR-UL112-3p及miR-UL112-5p水平明显高于CD8+细胞低水平组(P均<0.01);以CD8+细胞低水平组miR-UL112-3p、miR-UL112-5p水平为标准,CD8+细胞高水平组miR-UL112-3p、miR-UL112-5p阳性率分别为59.65%、64.91%。分别以1.09、1.52作为miRUL112-3p和miR-UL112-5p诊断HCMV较高水平潜伏感染状态的Cut-off值,敏感性分别为59.65%、64.91%,特异性分别为97.1%、97.1%。以非肿瘤组miR-UL112-3p、miR-UL112-5p水平的x珋+s(即5.24、6.63)作为诊断HCMV激发感染的标准,肿瘤化疗组miR-UL112-3p、miR-UL112-5p阳性率分别为50.00%、73.33%。结论 miRUL112-3p及miR-UL112-5p对HCMV潜伏和激发感染可能有一定的诊断价值。

Objective To investigate the levels of micro RNA（miR）-UL1 1 2-3p and 5p in human peripheral blood mononuclear cells,and to evaluate the significance of miR-UL1 1 2 in the diagnosis of human cytomegalovirus （HCMV） atent and active infections.Methods Human peripheral blood mononuclear cells were isolated from 92 non-cancer patients （57 patients with high-level HCMV specific CD8 ＋T lymphocyte and 35 patients with low-level HCMV specific CD8 ＋T lymphocyte）and 30 cancer patients receiving chemotherapy.HCMV DNA,miR-UL1 1 2-3p and miR-UL1 1 2-5p were determined by real time fluorescence quantitation polymerase chain reaction （PCR）.The receiver operating characteristic （ROC）curve was used to evaluate the diagnostic significance of HCMV latent infections.Results In cancer group,the levels of HCMV DNA,miR-UL1 1 2-3p and miR-UL1 1 2-5p were higher than those in non-cancer group （P〈0.05 ）.The levels of HCMV DNA,miR-UL1 1 2-3p and miR-UL1 1 2-5p in patients with high-level CD8 ＋ T lymphocyte were significantly higher than those in patients with low-level CD8 ＋T lymphocyte （P〈0.01 ）.As the levels of miR-UL1 1 2-3p and miR-UL1 1 2-5p in patients with low-level CD8 ＋T lymphocyte for standards,the positive rates in patients with high-level CD8 ＋T lymphocyte were 59.65% and 64.91%.As 1 .09 and 1 .52 for the cut-off values of miR-UL1 1 2-3p and miR-UL1 1 2-5p in HCMV high-level latent infection,the sensitivities were 59.65% and 64.91%, and the specificities were 97.1% and 97.1%.In the x~＋s （5.24 and 6.63）standard of miR-UL1 1 2-3p and miR-UL1 1 2-5p levels of non-cancer group,the positive rates of cancer group were 50.00% and 73.33%.Conclusions There might be diagnostic significance for HCMV latent and active infections by determining the levels of miR-UL1 1 2-3p and 5 p.