FECH基因rs1787997多态性与有氧耐力训练后左心室结构功能关联性研究

Association between the rs1787997 Polymorphism in FECH Gene and Left Ventricular Structure and Function in Men of Han Nationality in Northern China

目的:选取中国北方汉族男性亚铁螯合酶(FECH)基因rs1787997多态位点,与耐力训练前后左心室结构、功能指标进行关联性研究,探讨该多态位点作为预测有氧耐力训练后左心室结构、功能指标适应性改变的分子标记。方法:选取102名中国北方汉族男性健康受试者,以95%-105%个体无氧阈强度进行5 000米跑训练,每周3次,共18周,训练前后测定安静及递增负荷下左心室结构与功能等指标。使用PCR-RFLP和测序方法解析该基因多态性的分布特征,并进行该多态与上述生理指标进行关联性分析。结果:有氧耐力训练前,CG基因型受试者在安静状态下的室间隔收缩末厚度(IVSS)、左室后壁收缩末厚度(PWS)、在100W负荷下的心动周期(100W/T)均显著性高于CC型或GG型(P<0.05)。有氧耐力训练后,CG基因型受试者在150W负荷、恢复期时心动周期增加的幅度(△150W/T、△reT)均显著性高于GG型(P<0.05)。结论:在FECH基因rs1787997多态性中,CG基因型在左心室结构与功能的初始水平上面不仅具有较高的遗传优势,还具有较高的耐力训练敏感性,可以作为预测有氧耐力训练敏感性的分子标记。

Objective： To investigate the association between the rs1787997 polymorphism of FECH gene and left ventricular structure and function, and explore the feasibility that SNP rs1787997 has as genetic mark for predicting the effect of aerobic endurance training in men of Han nationality in Northern China. Methods ： 102 healthy young male soldiers of Han Nationality in Northern China were recruited to undergo 18 -week 5000m running with the intensity of 95% -105% anaerobic threshold with 3 times a week. Left ventricular structure and function parameters under different loads were measured before and after protocol. The PCR -RFLP and gene sequencing was used to analyze the distribution of this polymorphism. The association of the polymorphism with initial endurance capacity and endurance training response was analyzed. Results ： （ 1 ） Before 18 - week 5000m running, the initial mean value of the inter ventricular septal wall thickness systolic （IVSS）, left ventricular posterior wall systolic （PWS）, cardiac cycle （T） under 100W load in CG genotype were significantly higher than in CC or GG genotype（ P 〈 0.05 ）. （2）After 18 -week 5000m running, the increased degree of cardiac cycle（T） under 150W load and recovery in CG genotype was significantly higher than in GG genotype （ P 〈 0.05 ）. Conclusion ： In the rs1787997 polymorphism of FECH Gene, CC genotype has not only initial genetic advantage, but also higher training response in cardiac structure and function to aerobic training, and it could be the genetic marker in predicting endurance training response.