南高丛蓝莓奥尼尔快速增殖的研究

Rapid Propagation of Southern Highbush Blueberry O'Neal

为了探讨南高丛蓝莓奥尼尔快速增殖的条件,以奥尼尔无菌苗幼嫩茎段为试验材料,对增殖培养和叶片再生条件进行研究。结果表明,WPM（改良）＋0.5~1.0 mg/L ZT的增殖培养基能使增殖系数提高到5以上,且丛生苗生长健壮和生长速度快;降低盐浓度和添加玉米素是诱导叶片再生的关键;未切全叶的再生诱导优于切断叶片,前者在1/2 WPM＋2.0 mg/L ZT的培养基中的再生率高达71.4%,且主要形成具有2~4个芽的簇生苗。因此,将最佳的增殖培养和叶片再生相结合,使南高丛蓝莓奥尼尔可持续快速增殖,为进一步获得优良的奥尼尔种苗及其工厂化生产奠定重要基础。

To investigate rapid propagation in southern highbush blueberry O＇Neal, the conditions of propagation culture and leaf regeneration were optimized when young stem segments of aseptic seedlings were used as explants. The results indicated that the shoot propagation rate increased more than five times, moreover multiple shoots growing fast and robust, when WPM （improved） ＋ 0.5-1.0 mg/L ZT proliferation medium was used. The key conditions of leaf regeneration were the decrease of salt concentration and the addition of ZT. Leaf regeneration rates of a whole leaf were superior 71.4% and most of regenerative shoots with 2-4 sprouts, to that of a cut leaf, which of the former were up to when WPM （improved） ＋ 2.0 mg/L ZT proliferation medium was used. Thus, the optimum of propagation medium and leaf regeneration were successfully established in Southern highbush blueberry O＇Neal, which could realize continuous and rapid growth, establishing the important basis of good seedlings and factory production of O＇ Neal in the future.