汉族和维吾尔族结直肠癌患者K-ras基因突变情况及影响因素分析

Detection and Analysis on Influencing Factor of K- ras Gene Mutations in Han and Uyghur Patients with Colorectal Cancer

目的探讨结直肠癌患者K-ras基因突变情况,比较汉族与维吾尔族患者K-ras基因突变率的差异,同时分析K-ras基因突变的影响因素。方法收集2010年1月—2013年3月新疆医科大学第一附属医院结直肠癌活检或手术切除病理标本91例,汉族52例,维吾尔族39例。应用焦磷酸测序法对K-ras基因外显子2第12、13密码子突变情况进行检测,比较汉族与维吾尔族患者K-ras基因突变率的差异,采用单因素分析和多因素Logistic回归法分析K-ras基因突变的影响因素。结果 91例结直肠癌患者中K-ras基因突变型33例,总突变率为36.3%,单纯12密码子突变24例(占72.7%),单纯13密码子突变9例(占27.3%),无12、13密码子均突变病例。维吾尔族12密码子突变率明显高于汉族(P<0.05),但两组总突变率及13密码子突变率比较,差异均无统计学意义(P>0.05)。单因素分析和多因素Logistic回归分析显示,结直肠癌患者中K-ras基因突变率与性别、年龄、吸烟史、饮酒史、肿瘤部位、大体病理类型、分化程度、分期、浸润深度、淋巴结转移及远处转移均无相关性(P>0.05)。结论结直肠癌患者K-ras基因突变率高,维吾尔族12密码子突变率高于汉族。K-ras基因突变率与患者临床病理特征无明显相关性。

Objective To study the K-ras gene mutations in Han and Uyghur patients with colorectal cancer in Xin-jiang and to compare the K-ras gene mutation rate as well as to analyze influencing factors. Methods 91 cases of paraffin-embedded tissue specimens of colorectal cancer patients（52 cases of Han and 39 cases of Uyghur）admitted to the First Affiliated Hospital of Xinjiang Medical University from January 2010 to March 2013 were collected. K-ras gene mutations in codon 12 and 13 of exon 2 were detected by pyrosequencing. The K-ras gene mutation rate between Han and Uyghur was compared,and uni-variate and multivariate Logistic regression analysis were used to analyze influencing factors for K-ras gene mutation. Results 33 cases（36. 3%）were mutated at K-ras gene. 24 cases（72. 7%）were mutated at codon 12 and 9 cases（27. 3%）were mutated at codon 13,and no case had mutation at both codon 12 and codon 13. The mutation at codon 12 in Uyghur was significantly higher than in Han（P〈0. 05）,but the overall mutation rate and mutation rate at codon 13 showed no statistically significant difference（P〉0. 05）. The mutation rate of K-ras gene in patients with colorectal cancer had no correlation with sex, age,smoking history,drinking history,tumor location,pathologic type,degree of differentiation,stage,depth of invasion, lymph node metastasis and distant metastasis（P〉0. 05）. Conclusion K-ras gene mutation rate is high in patients with colorectal cancer. Mutation rate at codon 12 in Uyghur is higher than in Han. K-ras gene mutation rate in patients has no significant correlation with clinical and pathological features.