小型吸虫成虫永久性玻片标本制作技术

Preparation technique of permanent slide specimens for adult worms of small trematodes

目的探索小型吸虫成虫永久性玻片标本制作的理想技术,为寄生虫学实验教学和科学研究提供优质的标本资源。方法采用固定、染色、脱水、透明和封片的改进方法制作小型吸虫成虫染色标本。通过对染色(2,4,6 h)、分色(2,6,10min)、脱水(20,30,40 min)和透明时间(20,25,30 min)等步骤分别进行分组试验处理标本,获得整体玻片标本。结果经固定处理后的虫体通过染色2-4 h,分色6 min,脱水30 min,透明30 min后封片获得的永久性玻片标本效果最佳。运用本方法制作的小型吸虫成虫标本形态逼真、色泽鲜艳、内部结构清晰、组织器官层次分明、长期保存不褪色。结论染色、分色、脱水及透明的时间是小型吸虫成虫永久性玻片标本制作技术的关键。

Objective To explore an ideal preparation technique of permanent slide specimens for adult worms of small trematodes for providing the high quality samples or materials in experimental teaching and scientific research. Methods By the improved preparation method such as fixation, stain, dehydration, transparency and mounting, the permanent slide specimens for adult worms of Paragoni- mus and Clonorchis sinensis were obtained based on grouping tests such as dyeing time （2,4,6 h）, color separation time （2,6,10 min） ,dehydration time （20,30,40 min） and transparency time （20,25,30 min）. Results By means of 2 -4 h dyeing,6 min colour separation,30 min dehydration,30 min transparent,the dyeing effect of fixed adult worms was optimum. The slide specimens for adult worms of small trematodes made by this method shared good morphology, bright color, clear structure, distinct tissues and organs, and could be permanently preserved but did not fade. Conclusion The preparation keys of permanent slide specimens for adult worms of Paragonimus and Clonorchis sinensis are the time of dyeing, colour separation,dehydration and transparency.