AEG-1siRNA对宫颈癌细胞株增殖和凋亡影响的研究

Study on influence of AEG-1 siRNA on proliferation and apoptosis of cervical carcinoma SiHa cells

目的探讨siRNA下调星形细胞上调基因1(AEG-1)表达对宫颈癌细胞增殖和凋亡的影响。方法用设计合成的AEG-1siRNA转染宫颈癌SiHa细胞,采用荧光定量RT-PCR技术观察AEG-1siRNA对AEG-1基因表达的影响;用四甲基偶氮唑盐(MTT)法观察AEG-1siRNA对宫颈癌SiHa细胞增殖的抑制作用;用流式细胞术检测AEG-1siRNA对宫颈癌SiHa细胞凋亡和细胞周期的影响。结果 AEG-1siRNA转染人宫颈癌SiHa细胞后能显著下调AEG-1基因的表达,与对照组比较差异有统计学意义(P<0.01);同时AEG-1基因表达的下调可以显著抑制宫颈癌SiHa细胞的增殖(P<0.01),促进宫颈癌SiHa细胞凋亡(P<0.01),并将细胞周期阻滞在G0/G1期。结论 AEG-1siRNA可下调宫颈癌SiHa细胞AEG-1基因的表达,并抑制宫颈癌SiHa细胞的增殖,促进其凋亡。

Objective To investigate the effect of siRNA-down-regulating astrocyte elevated gene-1（AEG-1）on the proliferation and apoptosis of cervical carcinoma cells.Methods The designed and synthesized AEG-1 siRNA was transfected to the cervical car-cinoma SiHa cells.The influence of AEG-1 siRNA on the expression of AEG-1 gene was detected by real-time fluorescent quantita-tive-polymerase chain reaction （RT-PCR）;the inhibiting effect of AEG-1 siRNA on the proliferation of cervical cancer SiHa cells was detected by the MTT method;the influence of AEG-1 siRNA on apoptosis and cell cycle of the cervical cancer SiHa cellswas determined by the flow cytometry.Results Compared with the control group,AEG-1 siRNA transfecting human cervical cancer Si-Ha cells could evidently down-regulate the expression of AEG-1 gene with statistical difference （P〈0.01）;at the same time,the dwn-regulation of AEG-1 gene expression could significantly inhibit the proliferation of the cervical cancer SiHa cells （P〈0.01）, promote the apoptosis of SiHa cells （P〈0.01）and arrest the cell cycle in G0/G1 phase.Conclusion AEG-1 siRNA can down-regulate the expression of AEG-1 gene in the cervical carcinoma SiHa cells,inhibit the proliferation of the cervical carcinoma SiHa cells and promote their apoptosis.