PD-ECGF基因上调在PCI术后血管内膜损伤修复中的作用研究

Preliminary study on repairing effect of up-regulated PD-ECGF gene in intimal injury after PCI

目的：通过在兔颈动脉球囊损伤模型中转染血小板衍化内皮细胞生长因子（PD-ECGF）基因使其上调，探讨该基因在预防血管再狭窄中的作用。方法36只新西兰白兔（体质量2．5～3．0 kg）建立兔颈动脉球囊损伤模型，分为实验组（n＝18）和对照组（n＝18）：实验组使用病毒进行 PD-ECGF基因转染；对照组采用交付剂处理。3周后，采用 Evans蓝染色和 HE染色分别检测各组内皮覆盖率及新生内膜的改变情况；采用免疫组化技术和免疫印迹法检测颈动脉组织中 PD-ECGF蛋白表达情况。结果实验组内皮覆盖率[（72．8±4．0）％]显著高于对照组[（33．6±7．4）％]；HE染色可见实验组内膜及中层平滑肌增生程度较对照组明显减轻；免疫组化及免疫印迹法结果均表明实验组颈动脉组织中 PD-ECGF蛋白表达显著高于对照组（P＜0．05）。结论在家兔颈动脉球囊损伤模型中，上调PD-ECGF基因具有抑制平滑肌细胞增殖和促进内皮细胞生长以及血管生成的作用。

Objective To investigate the role of PD-ECGF gene in preventing the vascular restenosis by up-regulating the PD-ECGF gene in the rabbits′carotid artery balloon injury model.Methods 36 New Zealand white rabbits （body weight 2.5-3.0 kg） were established the rabbits′carotid artery balloon injury model and randomly divided into the experimental group （n=18）and the control group（n=18）.The experimental group was received the PD-ECGF gene transfection by virus and the control group was treated with the delivery agent.After 3 weeks,the intima coverage rate was tested by Evans blue staining.HE staining was used to investigate the morphological alteration of neointima and accurately measure the area of arterial intima.Finally,the expression of PD-ECGF protein was determined by the immunohistochemical technique and Western blot.Results The intima coverage rate in the experimental group was significantly higher than that in the control group （72.8±4.0%vs.33.6±7.4%,P〈0.05）;the HE staining showed that the hyperplasia degrees of intima and middle-lever smooth muscle in the experimental group were significantly alleviated than the control group;moreover,the immunohistochemical and Western blot results showed that the expression of PD-ECGF protein in the experimental group was significantly higher than that in the control group （P〈0.05）.Conclusion Up-regula-ting PD-ECGF gene has the role for inhibiting the proliferation of vascular smooth muscle cells and promoting the epithelial cell growth and angiogenesis in rabbits′carotid artery balloon inj ury model.