摘要
以十六烷基溴化铵(CTAB)为结构导向剂,正硅酸乙酯(TEOS)为硅源,在碱性环境下经过自组装过程对单分散性磁性Fe3O4纳米粒子进行包覆,制备出磁性硅基介孔纳米粒子Fe3O4@SiO2.结合X射线衍射、傅里叶变换红外光谱(FTIR)、透射电子显微镜(TEM)以及氮气吸附-脱附等技术对Fe3O4@SiO2粒子进行表征.结果表明Fe3O4@SiO2纳米粒子具有球形形貌,平均直径约为150 nm,蠕虫状介孔结构,比表面积为932 m2/g,孔径为2.5 nm且分布较均匀,包覆后Fe3O4的结构得以保持,同时材料具有很好的磁响应能力.以抗癌药紫杉醇(Paelitaxel,TXL)为模型药物进行负载,实验结果表明,Fe3O4@SiO2对TXL的负载能力为80 mg/g,TXL-Fe3O4@SiO2对TXL的缓释时间持续120 h以上,累积释放量达到30 mg/g.通过噻唑蓝比色(MTT)法测量了TXL-Fe3O4@SiO2粒子对体外培养的HeLa细胞的细胞毒性,与相同浓度的TXL相比,TXL-Fe3O4@SiO2对HeLa细胞的抑制率明显增高.
Magnetic Fe3O4@SiO2 mesoporous silica nanoparticles (MSNs) were synthesized with Fe3O4 nanoparticles as crystal core, cetyhrimethylammium bromide (CTAB) as structural directional agent through the hydrolysis of tetraethoxysilane (TEOS) and self-assembly procedure under the basic conditions. The synthe- sized MSNs were characterized via the XRD, FTIR, TEM and N2 adsorptiom-desorption. The results show that these MSNs have high sepcific surface area of 932 cm2/g, particles size of 150 nm and typical hexagonal packing mesoporous structure. Simultaneously, all Fe3O4 nanoparticles are perfectly embedded in silica bulk. As a TXL carrier, Fe3O4@SiO2 exhibits good adsorption with the maximum adsorption capacity of 80 mg/g and TXL-Fe3O4@SiO2 has a more than 120 h sustained release for TXL with accumulative quantity of 30 mg/ g. While the release behaviour is controlled by various pH values. Higher the pH is, larger the accumulative release is. Experiments of cytotoxicity on HeLa cells indicate that the Fe3O4@SiO2 particles exhibit excellent biocompatibility. TXL-Fe304@ SiO2 shows significant inhibition effect on HeLa cells than bare TXL under the same drug concentration.
出处
《高等学校化学学报》
SCIE
EI
CAS
CSCD
北大核心
2014年第7期1509-1514,共6页
Chemical Journal of Chinese Universities
基金
国家自然科学基金(批准号:30800219)
中国矿业大学理科专项基金(批准号:LK0303)
江苏省介入医疗器械研究重点实验室开放基金(批准号:jr1105)资助~~