马齿苋籽油脂肪酸组成的GC-MS分析

Fatty acid composition of Portulaca oleracea seeds oil with GC-MS

利用索氏抽提器和石油醚溶液提取马齿苋籽油,出油率为16.8%±0.13%。皂化和甲基化得到总脂肪酸甲酯经GC分析,马齿苋籽油检出7种成分。经GC-MS联机分析,采用面积归一化法确定马齿苋籽油脂肪酸成分及其相对百分含量为棕榈酸(12.00%±0.60%)、α-亚麻酸(82.35%±0.64%)、硬脂酸(3.94%±0.10%)、花生酸(0.74%±0.05%)、二十烯酸(0.21%)、山萮酸(0.56%±0.03%)、木蜡酸(0.31%±0.03%)。结果显示,马齿苋籽油不饱和脂肪酸含量丰富,相对百分含量高达82.56%±0.64%,主要由α-亚麻酸组成,将其开发成为药食两用功能性食品,具有良好应用前景。

The use of petroleum ether by Soxhlet get Portulaca oleracea seed oil. The extracted yield of Portulaca oleracea seed oil was 16.8%±0.13%. Fatty acids compositions were determined from Portulaca oleracea seed oil by saponification,methylation and GC-MS. The results indicated that the oil from Portulaca oleracea seed was composed of 7 fatty acids. The results of GC-MS and area normalization method showed that the Portulaca oleracea seed oil mainly contained Palmitic acid （12.00%±0.60%）, Linolenic acid （82.35%±0.64%）, Stearic acid （3.94% ±0.10% ）, 11 -Eicosenoic acid （0.21% ）, Eicosanoic acid （0.74% ±0.05% ）, Behenic acid （0.56% ±0.03% ）, Lignoceric acid （0.31%±0.03%）. While unsaturated fatty acids composition （82.56%±0.64%） from Portulaca oleracea seed oil were mainly composed of linolenoic acid. Experimental results indicated that the Portulaca oleracea seed oil was a kind of functional oil which had abundant n-3 unsaturated fatty acids,with high nutritional value and phanmcotherapy efficacy.